摘要
目的通过分析豚鼠外周血单个核细胞(Peripheral blood mononuclear cell,PBMC)与耳蜗组织中糖皮质激素受体(glucocorticoid receptor,GR)mRNA和蛋白含量变化,探讨PBMC与耳蜗组织中GR含量的相关性。方法将32只健康白色豚鼠随机分为地塞米松组和对照组,地塞米松组予地塞米松(5mg/ml,10mg/kg/day)腹腔连续注射7天,对照组予等体积的生理盐水腹腔连续注射7天。给药结束后次日取豚鼠PBMC及双侧耳蜗组织,通过实时荧光定量PCR检测豚鼠PBMC和左耳耳蜗组织中GR mRNA表达水平,通过Western blot结果半定量分析豚鼠PBMC和右侧耳蜗组织中GR蛋白的含量,并运用统计学软件SPSS16.0进一步分析豚鼠PBMC与耳蜗组织中GR mRNA及GR蛋白表达量的相关性及短期全身使用地塞米松对其表达量的影响。结果 GR mRNA与GR蛋白在豚鼠PBMC与耳蜗组织中均有表达,地塞米松组PBMC及耳蜗组织中GR mRNA与GR蛋白含量较对照组有明显提高,统计学相关性分析结果提示地塞米松组及对照组的PBMC与耳蜗组织的GR mRNA、GR蛋白表达量存在正相关,结果有统计学意义。结论豚鼠短期全身使用地塞米松可以使PBMC与耳蜗组织中的GR mRNA、GR蛋白含量均同步上调,豚鼠PBMC与耳蜗组织中GR mRNA、GR蛋白的含量变化具有正相关性。PBMC中GR含量可间接反映耳蜗组织的GR表达水平。
Objective To study correlation of glucocorticoid receptor （GR） contents between peripheral blood mononuclear cells （PBMC） and the cochlea in guinea pigs by measuring the expression of GR mRNA and GR protein and the effects of systemic injection of dexamethasone on the expression level. Methods Healthy guinea pigs （n=32） were randomly divided into two groups （dexamethasone treatment and control groups）. Dexamethasone （5 mg/ml, 10 mg/kg/day） was injected intraperitoneally in the 15 guinea pigs of the dexamethasone group for 7 continuous days, while same volume physiological saline was injected intraperitoneally in the 17 guinea pigs of the control group. PBMCs and bilateral cochlear tissue were collected. Real time PCR and Western blot were used to detect the expression level of GR mRNA and GR protein in PBMCs and cochlear tissues.The data were analyzed with the SPSS 16.0 software. Results GR mRNA and GR protein were expressed in both PBMCs and cochlear tissues. The expression levels of GR mRNA and GR protein increased significantly in the dexamethasone treatment group and showed close correlation between PBMCs and the cochlea . Conclusions The levels of GR mRNA and GR protein in the PBMC indirectly reflect those in the cochlea in guinea pigs.
出处
《中华耳科学杂志》
CSCD
北大核心
2012年第1期60-64,共5页
Chinese Journal of Otology
基金
2009年江苏省"六大人才高峰"项目
南京医学科技发展项目(WKK09105)
关键词
受体
糖皮质激素
外周血单个核细胞
耳蜗
豚鼠
Receptors
Glucocorticoid
Peripheral blood mononuclear cell
Cochlea
Guinea pig
