摘要
目的:测定山茱萸及其炮制品中5-羟甲基糠醛和没食子酸的含量。方法:色谱条件,Kromasil-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(5∶95),流速1.0 mL.min-1,检测波长240 nm,柱温30℃。测定没食子酸的色谱条件,Kromasil-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.1%磷酸(8∶92),流速1.0 mL.min-1,检测波长271 nm,柱温30℃。结果:与生品相比,炮制品中5-羟甲基糠醛为新增加成分,没食子酸含量明显增加;高压蒸制中两种成分含量高于常压蒸制,但没有显著性差异。结论:该方法稳定可靠、简便可行,可以为制定山茱萸的炮制工艺提供依据。
Objective：To compare the content of 5-hydroxymethyl furfural and gallic acid in the raw medical material and the different processed products of Cornus officinalis.Method： Kromasil-C18（4.6 mm×250 mm,5 μm） was used.The mobile phase was acetonitrile-water（5∶95）,detection wavelength was at 240 nm for 5-hydroxymethyl furfural and methanol-0.1% phosphoric acid（8∶92）,and detection wavelength was at 271 nm for gallic acid,respectively.The flow rate was 1.0 mL·min-1 and the column temperature was at 30 ℃.Result： Compared to the C.officinalis,5-hydroxymethyl furfural was a new competent and gallic acid was significantly increased.The content of 5-hydroxymethyl furfural and gallic acid in high-pressure processed products were higher than traditional pressure products,but there was no significantly difference.Conclusion： The HPLC method developed for determination of 5-hydroxymethyl furfural and gallic acid from different processed products of C.officinalis is simple and valid.This study can provide a scientific basis for establishing rational preparing process.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期64-67,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"攻关课题(2006BAI09B06-06)
关键词
山茱萸
炮制
5-羟甲基糠醛
没食子酸
Cornus officinalis
processed
5-hydroxymethyl furfural
Gallic acid
