摘要
目的:观察芦荟及其复方提取物对小鼠巨噬细胞炎症因子的影响。方法:RAW264.7细胞按2×105/mL稀释,100μL/孔接种入96孔板,药物质量浓度为250,125,62.5 g.L-1下采用噻唑蓝比色法(MTT法)作用4 h,检测芦荟及其复方提取物对小鼠巨噬细胞的毒性作用;药物质量浓度为50,25,12.5 g.L-1下采用硝酸还原酶法作用24 h,测定小鼠巨噬细胞一氧化氮(NO)含量,并用酶联免疫吸附法(ELISA法)测定其在单纯药物及脂多糖(LPS)1 mg.L-1诱导情况下小鼠巨噬细胞肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)的含量。结果:芦荟及其复方提取物具有促进巨噬细胞释放NO的作用,并与药物浓度成正相关(P<0.01);芦荟有促进TNF-α分泌(P<0.01)和抑制IL-10分泌(P<0.05)的作用,其复方在LPS诱导下则对TNF-α和IL-10都有抑制作用(P<0.01)。结论:芦荟在250～7.8 g.L-1下对巨噬细胞无细胞毒性,其复方则有一定毒性;芦荟及其复方对小鼠巨噬细胞炎症因子的释放有一定影响。
Objective：To observe effect of the Aloe vera and its compound extract on inflammatory factors.Method： Cells diluted by 2×105/mL were inoculated into 96-well plates,100 μL per well,concentrations of druds was 250,125,62.5 g·L-1,MTT method was assay to test the toxic effects of macrophages.When the concentrations was 50,25,12.5 g·L-1 macrophage nitric oxide（NO） levels was measured.Enzyme-linked immunosorbent assay（ELISA） were used to measure tumor necrosis factor-α（TNF-α） and interleukin-10（IL-10） levels in pure drug and lipopolysaccharide（LPS）-induced murine macrophage.Result：Aloe vera and its compound extract promoted releasing of NO,with a concentration dependent manner（P0.01）;Aloe vera could promote TNF-α secretion（P0.01） and inhibition of IL-10 secretion（P0.05）,the compound inhibited LPS induced releasing of TNF-α and IL-10（P0.01）.Conclusion： Aloe in 250-7.8 g·L-1 concentration has non-cytotoxic macrophages,compound extract has a certain toxic action,and can exert some influence on release of inflammatory factors.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期166-169,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑计划项目(2008BAI53B09)
江苏高校优势学科建设工程资助项目
南京中医药大学中西医结合优势学科
关键词
炎症
芦荟
小鼠巨噬细胞
细胞因子
Inflammation
Aloe vera
mouse macrophage cells
cytokines
