摘要
目的:比较补肾解毒活血方与益气补血方预防环磷酰胺(CTX)引起骨髓抑制小鼠造血功能的效果及作用机制,为临床合理配伍提供依据。方法:清洁级昆明种小鼠80只,随机分为正常对照组、模型组、补肾解毒活血组和益气补血组,中药组分别给予25.55,17.47 g.kg-1.d-1中药灌胃,正常对照组和模型组给予等体积的生理盐水,每日灌胃1次,连续给药10d,第8天时采用腹腔注入环磷酰胺(100 mg.kg-1.d-1),连续造模3 d。各组随机抽取10只,在第7天(造模前1 d)及造模完成后的第1,3,5,7,10天做外周血象检测,剩余10只于造模后第1天进行骨髓有核细胞计数(BMNC)、粒系、红系造血祖细胞(CFU-GM,CFU-E和BFU-E)集落形成单位、细胞凋亡检测及血清粒-巨噬细胞集落刺激因子(GM-CSF)及促红细胞生成素(EPO)检测。结果:各组小鼠白细胞和血小板数分别于造模后第1天和第5天降至最低,并分别于第5天和第10天基本恢复至正常,但益气补血组下降程度较模型组轻(P<0.01),而补肾解毒活血组恢复明显早于模型组和益气补血组(P<0.05);造模后模型组BMNC,CFU-GM及BFU-E显著下降(P<0.01),且两中药组均明显高于模型组(P<0.01),其中BFU-E以补肾解毒活血组为优(P<0.05);造模后小鼠骨髓细胞凋亡率明显增加(P<0.01),约是正常组的14.7倍,两中药组骨髓细胞凋亡率均明显低于模型组(P<0.01);造模后GM-CSF较正常组显著降低(P<0.01),但两中药组GM-CSF明显高于模型组(P<0.05)。各组血清EPO水平较正常组没有明显差异。结论:预防给予益气补血药或补肾活血解毒药均可以加快化疗后骨髓抑制白细胞的恢复,尤以益气补血方为优;益气补血方减轻血小板下降程度更好,补肾活血解毒方促使血小板恢复更佳。预防给予两组中药均可减轻小鼠环磷酰胺造成的BMNC和CFU-GM下降程度,从而有利于白细胞的恢复;两组中药不是通过提高血清促红细胞生成素的水平,而是通过减轻环磷酰胺造成的BFU-E的减少,达到保护红系造血的效果,以补肾解毒活血方为优。预防应用补肾解毒活血药与益气补血药可以明显降低环磷酰胺引起的骨髓细胞凋亡,似以益气补血组为佳。
Objective：To investigate the effect and mechanism of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe on preventing bone marrow suppression induced by cyclophosphamide（CTX） in mice,and provide the basis for clinical compatibility.Method： Eighty Clean grade Kunming mice were used and randomly divided into 4 groups： normal control group,model group,Bushen Jiedu Huoxue group,Yiqi Buxue group.The mice were administrated normal saline,Bushen Jiedu Huoxue Recipe or Yiqi Buxue Recipe once a day continually for 10 days hrough gastric tue.Establish the experimental model of bone marrow suppression at day 8 by ip CTX 100 mg·kg-1,3 days continuously.Peripheral blood cells were counted at day7（the day before the CTX injection）and 1,3,5,7,10 days after the successful model set.;bone merrrow nucleated cell（BMNC）、 colony-forming unit-granulocyte-macraphge（CFU-GM）、 colony-forming unit-erythroyte（CFU-E） and burst-forming unit-erythocyte（BFU-E） were counted 1 day after the successful model set.Result： White blood cell and platelet counts were down to the minimum at day 1 and day 5 after modeling,respectively,and recovered to normal at day 5 and day 10.The number of BMNC,CFU-GM and BFU-E in Yiqi Buxue group and Bushen Jiedu Huoxue group were lower than the normal group,but much higher than that of model group（P0.01）.The ratio of Apoptosis of model group was significantly increased（P0.01）,which is about 14.7 times of normal control group.The other two groups were decreased obviously than model group（P0.01）.GM-CSF was significantly decreased（P0.01） after modeling,however Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe could greatly increase it（P0.05）.The serum EPO levels didn＇t show much difference.Conclusion： Preventively use of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe could accelerate the recovery of white blood cell and platelet,and Bushen Jiedu Huoxue Recipe was better on promoting platelet to recovery.Both of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe can reduce the decrease of BMNC,CFU-GM and BFU-E,which was better in Bushen Jiedu Huoxue group;and can less the ratio of apoptosis of bone marrow cells,which was better in Yiqi Buxue group.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期242-245,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30973824)
关键词
补肾解毒活血方
益气补血方
环磷酰胺
骨髓抑制
Bushen Jiedu Huoxue Recipe
Yiqi Buxue Recipe
cyclophosphamide
bone marrow suppression
