摘要
目的 :建立固相微萃取 (SPME)和GC/NPD分离、分析尿中痕量毒鼠强方法。方法 :1ml尿样以 16 0 5为内标 ,采用 85μmPA萃取头浸入萃取 ,吸附 2 0min ,热解吸 3min ,于GC/NPD测定。 结果 :方法的线性范围为 10～ 5 0 0ng·ml-1尿 ,最低检出浓度小于 10ng·ml-1,RSD小于 10 %。用该法监测了 1例毒鼠强中毒幼儿尿中毒鼠强含量 ,在中毒后 3个月内仍从尿中检出毒鼠强成分 ,与GC/MS结果一致。结论 :方法快速、简便、可靠 ,适用于临床和法庭毒物分析。
OBJECTIVE:A solid phase microextraction(SPME) and GC/NPD method for the isolation and determination of tetramine in human urine was established.METHODS:1 ml urine,using 1605 as the internal standard,was extracted with SPME fiber (85 μmPA) after immersing SPME fiber in the sample for 20 min.Tetramine on the fiber was desorbed by exposing the fiber in the GC injector for 3 min.RESULTS:The linear range of the spiked urine samples was over 10～500 ng·ml -1 (r=0.9988) with RSD less than 10%.The limit of detection by SPME for the tetramine in urine was 10 ng·ml -1 .Tetramine was found in the urine samples of rodenticide poisoned 10 months old baby 3 months after intoxication using this method,which was comparable to the results by GC/MS. CONCLUSION:The method is simple,rapid and accurate.It is suitable for the identification of tetramine in the rodenticide posioned victim in the medical science and forensic science.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2000年第5期341-343,共3页
Chinese Pharmaceutical Journal
