摘要
运用行为学方法通过金属离子诱发神经毒性,建立阿尔海默茨症动物模型。通过运动行为观察、应激回避条件模型检测在pH值为7.8、6.8、5.8条件下暴露铝离子24h和96h后铝离子对成年斑马鱼运动行为和学习记忆能力的作用,探讨金属元素在酸性环境下诱发神经毒性导致阿尔海默茨症与运动行为、学习记忆的关系。结果表明,pH5.8铝离子组暴露96h的运动行为活性和学习记忆能力与pH7.8铝离子组和pH6.8铝离子组相比有较显著变化。同时,pH5.8铝离子组暴露96h运动行为活性和学习记忆能力与pH5.8铝离子组暴露24h相比出现明显降低。这些都表明,铝在酸性环境下,与pH相互作用影响斑马鱼的运动行为与学习记忆能力,可能造成斑马鱼大脑关于记忆功能区域出现损伤,诱发神经毒性产生类似阿尔海默茨症发病症状。
We investigated the contribution of locomotor activity and learning and memory abilities to Alzheimer＇s disease induced by metal neurotoxicity. The metal neurotoxicity model was established by using locomotor activity tests and activity-avoidance conditioning paradigms, and observing changes in zebrafish behaviors after exposure to 50 μg/L of AlCl3 with different pH values （pH 7.8, pH 6.8 and pH 5.8） for 24 hours and 96 hours. The results showed Alzheimer＇s disease-like behavior in locomotor activity tests and activity-avoidance conditioning paradigms by zebrafish receiving AlCl3 in pH 5.8 significantly decreased compared to the control group and groups receiving AlCl3 in pH 7.8 and pH 6.8 for 96 hours and 24 hours. These results provide evidences that exposure to AlCl3 in an acid environment can influence locomotor activity and learning and memory abilities in zebrafish. In addition, exposure time and concentration of Al induced neurotoxicity and damaged the brain area of memory, resulting in Alzheimer＇s disease-like behavior.
基金
苏州市医学基础研究项目 (YJS0904)
关键词
铝
酸性环境
运动行为
学习记忆能力
阿尔海默茨症
Aluminum
Acid environment
Locomotor activity
Learning and memory abilities
Alzheimer＇s disease
