摘要
目的建立珠芪复肝颗粒中珠子草的鉴别和没食子酸的含量测定方法。方法采用薄层色谱法对珠子草进行定性鉴别。采用高效液相色谱法测定没食子酸的含量,以C18柱分离,柱温为室温,甲醇-0.1%磷酸水溶液(10∶90)为流动相,检测波长为270 nm,流速1.0 mL/min。结果薄层色谱中能鉴别出珠子草。没食子酸的线性范围为0.003 3～0.025 0μg(r=0.999 9),平均回收率为100.08%(RSD=1.24%,n=5)。结论所建立的方法简便、重复性好,可用于珠芪复肝颗粒中珠子草的鉴别和没食子酸的含量测定。
Objective To establish the methods for the identification of Herba Phyllanthus niruri L.and content determination of gallic acid in Zhuqi Fugan granules.Methods TLC was used for the identification of Herba Phyllanthus niruri L..HPLC was applied for the determination of gallic acid,using a column of C18.The column temperature was at room temperature,with methanol-0.1% phosphoric acid as amobile phase.The flow rate was 1.0 mL/min,and the detection wavelength was at 270 nm.Results Herba Phyllanthus niruri L.could be identified by TLC.The linear range of gallic acid was 0.003 3-0.025 0 μg（r=0.999 9） with the average recovery of 100.08%（RSD=1.24%,n=5）.Conclusion The established methods are simple and reproducible,and can be used for the identification of Herba Phyllanthus niruri L.and content determination of gallic acid in Zhuqi Fugan granules.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第4期47-48,共2页
Chinese Journal of Information on Traditional Chinese Medicine
