摘要
目的探讨冬虫夏草提取物抑制大鼠移植动脉硬化的效果及其机制。方法制备大鼠腹主动脉移植模型，分为4组进行实验：同系对照组供、受者均为Lewis大鼠，生理盐水灌胃60d；同种对照组，以Brown—Norway大鼠（BN大鼠）为供者，Lewis大鼠为受者，生理盐水灌胃60d；低剂量实验组，以BN大鼠为供者，Lewis大鼠为受者，以冬虫夏草提取物（1．5g·kg^-1·d^-1）灌胃60d；高剂量实验组，以BN大鼠为供者，Lewis大鼠为受者，以冬虫夏草提取物（3．0g·kg^-1·d^-1）灌胃60d。于移植后60d取移植动脉，HE染色，进行病理学观察；免疫组织化学和蛋白质印迹法检测血管内皮生长因子（VEGF）和血小板衍生生长因子BB（PDGF-BB）在移植动脉中的表达。酶联免疫吸附试验检测受鼠血清中VEGF和PDGF-BB含量。结果同系对照组移植动脉形态正常；同种对照组移植动脉呈移植物血管病表现，血管内膜显著增厚；2个实验组移植动脉呈内膜炎症改变，内膜厚度与同种对照组相比，差异有统计学意义（P〈0．05）。同种对照组移植动脉中VEGF和PDGF-BB的表达高于同系对照组（P〈0．05）；2个实验组移植动脉中VEGF和PDGF-BB的表达低于同种对照组（P〈0．05）。同系对照组受鼠血清中几乎无VEGF和PDGF-BB；同种对照组血清中VEGF和PDGF-BB浓度高于同系对照组（P〈0．05）；和同种对照组相比较，2个实验组血清VEGF和PDGF-BB浓度较低（P〈0．05）。结论冬虫夏草提取物能明显抑制动脉内膜的增生，可缓解慢性排斥反应所致的移植动脉硬化，这种保护作用可能与下调VEGF和PDGF-BB表达有关。
Objective To investigate the effects and mechanism of Cordyceps sinensis on transplant arteriosclerosis in a rat model. Methods Brown Norway aortic allografts were transplanted into Lewis recipient rats, and the recipient rats were randomly divided into four groups： group A, isograft control group, Lewis-Lewis （n = 10）; group B, allograft control group, BN Lewis （n = 10）. groups C and D, allograft experimental groups, BN Lewis （n = 10）. Rats were given normal saline via intragastric injection, once every day for 60） days in groups A and group B, and received different doses （1.5 and 3 g·kg^-1·d^-1） of Cordyceps sinensis in groups C and group D respectively. Grafts were harvested on the day 60 after transplantation. Intimal thickness was detected by HE staining. Protein was extracted from the abdominal aortas for Western blotting. Cellular localization was assessed by histology and immunohistochemistry. The serum was analyzed by an enzyme-linked inwnunosorbent assay （ELISA）. Results Transplanted arteries were normal in group A. Transplanted arteries in group A had allograft vasculopathy, and intimal thickness was significantly increased. Transplanted arteries in allograft experimental groups had endometritis changes, and intimal thickness was significantly decreased as compared with that in group B （P 〈 0. 05）. Immunohistochemistry and Western blotting revealed that the expression levels of VEGF and PDGF- BB proteins in group A were significantly higher than in group B, and those in groups C and D were significantly reduced as compared with group B （P〈0. 05）. ELISA showed that serum VEGF and PDGF-BB concentrations in group B were significantly increased as compared with group A （P 〈0. 05）. Serum VEGF and PDGF-BB concentrations were significantly reduced in groups C and D as compared with group B （P〈 0.05）. Conclusion Cordyceps sinensis could significantly inhibit the intimal hyperplasia, and delayed transplant arteriosclerosis caused by chronic rejection, which may be related to the down-regulated expression of VEGF and PDGF-BB.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2012年第4期236-240,共5页
Chinese Journal of Organ Transplantation
基金
浙江省中医药科技计划项目（2007CA082）
关键词
大鼠
主动脉
腹
移植
冬虫夏草
移植物闭塞
血管
Rats
Aorta, abdominal
Transplantation
Cordyceps sinensis
Graft occlusion, vascular
