摘要
目的:建立高效液相色谱法测定盐酸左旋咪唑中的有关物质。方法:色谱柱为Aichrombond C18(250 mm×4.6 mm,5μm);流动相为0.5%磷酸二氢铵(用三乙胺调节pH至6.5)-乙腈(70∶30);流速为1.0 ml.min-1;检测波长为215 nm;进样量为10μl。结果:有关物质各杂质峰与主峰之间分离良好,杂质2,3-二氢-6-苯基咪唑[2,1-b]噻唑盐酸盐的线性范围为1.11～11.10μg.ml-1(r=0.998 5),最低检测限为0.6 ng。结论:该方法专属性强、重复性好,可用于盐酸左旋咪唑的有关物质测定。
Objective： To establish an HPLC method for the determination of the related substances in levamisole hydrochloride.Method： The column was Aichrombond C18（250 mm×4.6 mm,5 μm）,the mobile phase consisted of 0.5% ammonium dihydrogen phosphate（adjusting pH to 6.5 with triethylamine）-acetone（70∶ 30） at the flow rate of 1.0 ml·min-1,the detection wavelength was 215 nm and the injection volume was 10 μl.Result： The linear range of 2,3-dihydrogen-6-phenylimidazol thiazoli hydrochloride was 1.11~11.10 μg·ml-1（r=0.998 5）,and the detection limit was 0.6 ng.Conclusion： The method is specific and reproducible for the determination of the related substances in levamisole hydrochloride.
出处
《中国药师》
CAS
2012年第4期512-514,共3页
China Pharmacist
