摘要
目的：探讨支气管和肺泡灌洗液（ＢＡＬＦ）脱氧核糖核酸（ＤＮＡ）和肺癌相关抗原（ＬＣＡ）检测对肺癌的诊断价值。方法：对肺癌组（５７ 例）和对照组（２９ 例）患者的病变部位进行支气管和肺泡灌洗，将回收的ＢＡＬＦ分别用流式细胞仪（ＦＣＭ）进行ＤＮＡ分析和用多株肺癌特异性单克隆抗体及金标记免疫斑点法检测ＬＣＡ。结果：以异倍体细胞阳性为诊断肺癌的标准，敏感性为４５．６１％ ， 特异性为９３．１０％ ，正确性为９２．８６％ 。以ＬＣＡ 阳性为肺癌的诊断标准，敏感性为７０．１８％ ，特异性为７５．８６％ ，正确性为８５．１１％ 。若以异倍体细胞和ＬＣＡ均呈阳性为肺癌的诊断标准，敏感性为４２．１１％ ，特异性下正确性均为１００％ 。结论：ＢＡＬＦ细胞ＤＮＡ分析和ＬＣＡ检测均对肺癌有辅助诊断价值。若临床上怀疑肺癌，ＢＡＬＦ检测又同时发现异倍体细胞和ＬＣＡ阳性。
Objective:To evaluate the diagnostic value of both cellular DNA analysis and lung carcinoma related antigen (LCA) test of bronchoalveolar lavage fluid (BALF) in patients with pulmonary cancer.Method:Both Cellular DNA analysis and LCA test of BALF from both 57 patients with lung carcinoma and 29 patients with noncancer was separately determined by flow cytometer(FCM) and by specific monoclonal antibodies of pulmonary multicarcinostrains antibody with gold marked immunoblot test Result:The aneuploidy was used as a diagnostic carcinocriterion,the sensitivity was 45.86%.The positive LCA was used as a liagnostic criterion,the sensitivity was 70.18%,the specificity was 93.10% and the exactitiud was 85.11%if,the specificity was 75.86% and the exactitud was 92.86%. the presence of aneuploidy together with the positive LCA was employed as the diagnostic criterion,the sensitivity was 42.11%,the specificity and the exactitude were separately 100%.Conclusion:These results suggest that both the DNA analysis and the LCA test of BALF have assistant diagnostic value to lung cancer.If the patients conditions are suscepted to carcinoma,the diagnosis of lung cacinoma may be considered when both the presence of aneuploidy with the positive LCA in BALF are found.
出处
《河北医学》
CAS
2000年第1期17-20,共4页
Hebei Medicine
关键词
肺肿瘤
DNA
LCA
支气管
肺泡灌洗液
DNA analysis
Lung cancer related antigen
Bronchoalveolar lavge fluid
Lung Carcinoma Diagnosis
