摘要
利用两个直链淀粉含量相似的水稻品种93-11和日本晴为亲本,采取单粒传法创建由190个家系组成的重组自交系群体,并构建了包含202个SSR、CAP和STS标记的遗传连锁图谱。采用复合区间作图法,在两个不同生态环境下(三亚、合肥)对糙米蛋白质含量和精米蛋白质含量的QTL进行了定位分析,共定位到9个QTL。其中,qBRPC1.3在两个环境中均被检测到,稳定性较高,其贡献率达9.4%;位于第1染色体的RM578-RM10596是多效性区间,同时控制糙米蛋白质含量和精米蛋白质含量,贡献率分别为6.4%和5.7%。
Based on single seed descend method a recombinant inbred lines population consisting of 190 lines was created by crossing two rice varieties with similar apparent amylose content, Nipponbare （japonica） and 93-11 （indica） by QTLs for brown rice protein content（BRPC） and milled rice protein content （MRPC） with a genetic linkage map including202 SSR, CAP and STS markers were identified. By composite interval mapping method in two environments sites （Sanya Hainan Province, China and Hefei Anhui Province, China）, a total of nine QTLs were identified, indicating that rice protein content was controlled by multiple genes, qBRPC1. 3 was detected at the two sites with high stability, explaining 9. 4% of total variation. The interval RM578-RM10596 on chromosome 1, controlled BRPC and MRPC, exDlaining 6.4% and 5.7 % of total variation, respectively.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第3期351-355,共5页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31071401)
科技部转基因重大专项(2008ZX08001-002
2009ZX08001-013B)
安徽省优秀青年基金资助项目(10040606Y03)
国家863计划资助项目(2012AA101102-4)
