摘要
为揭示浓香型特色烟叶形成的生理生化基础,采用质外体提取等方法分别测定了具有浓香型特色的烤烟品种NC89和对照品种89112烟叶衰老过程中的氮素代谢产物和相关酶活性的变化,并计算了氨气补偿点。结果表明,与89112相比,NC89品种叶片总氮和可溶性蛋白含量较高,在衰老过程中谷氨酰胺合成酶(GS)活性下降幅度小,谷氨酸脱氢酶(GDH)活性低;质外体铵离子浓度和质外体pH变化相对平缓,氨气挥发高峰期氨气补偿点偏低,氨气挥发潜力较小,最终总氮减少量较小。
To investigate the physiological and biochemical traits in the formation of robust flavor style, apoplast extraction was used to assay the variations of nitrogen metabolites and the activity of related enzymes during leaf senescence and the ammonia compensation point was calculated in flue-cured tobacco cv. NC89 and cv. 89112 （the control）, which possessed typical robust flavor style. The results showed that： comparing with 89112, NC89 contained more total nitrogen and soluble protein, during senescence stage, it displayed less decline in glutamine synthetase （GS） activity, lower glutamate dehydrogenase （GDH） activity, relatively flattened apoplastic NH4^＋ concentration and pH variations, and less reduction of total nitrogen due to lower ammonia emission potential indicated by lower ammonia compensation point at the peak volatilized stage.
出处
《烟草科技》
EI
CAS
北大核心
2012年第5期65-68,共4页
Tobacco Science & Technology
基金
川渝中烟工业公司博士后项目"凉攀地区红花大金元合理群体结构研究及其遗传改良"(川渝烟工技研[2010]347号-20)
关键词
浓香型
烤烟品种
衰老
氮素
氨气补偿点
谷氨酰胺合成酶
谷氨酸脱氢酶
Robust flavor style
Flue-cured tobacco cultivar
Senescence
Nitrogen （N）
NH3 compensation point
Glutamine synthetase
Glutamate dehydrogenase
