摘要
背景:血管细胞黏附分子1基因表达产物在动脉粥样硬化发生过程中扮演重要角色;补阳还五汤具有改变血液流变学的性质、降低血脂、抗动脉硬化的功效。目的:观察补阳还五汤对动脉粥样硬化模型的治疗作用,并在分子水平探讨其作用机制。方法:高脂饮食加维生素D3诱导大鼠动脉粥样硬化模型。32只造模成功的大鼠随机摸球法均分为4组干预:补阳还五汤组、川芎嗪组、模型组和正常对照组。观察各组血脂水平及主动脉中血管细胞黏附分子1mRNA的表达。结果与结论:造模成功率为92%。各组血脂水平比较:补阳还五汤组指标接近正常,与其他两组比较,差异有显著性意义。各组血管细胞黏附分子1mRNA表达比较:正常对照组无表达,补阳还五汤组显著低于模型组和川芎嗪组。说明益气活血代表方剂补阳还五汤可以下调主动脉组织血管细胞黏附分子1mRNA的表达,降低血脂,具有抗动脉粥样硬化作用,其机制可能与降低主动脉组织血管细胞黏附分子1mRNA的表达有关。
BACKGROUND：Gene expression product of vascular cellular adhesion molecule-1（VCAM-1） mRNA plays an important role in atherosclerosis.Bu Yang Huan Wu Tang has the effect of changing the quality of blood rheology,decreasing the level of blood-lipid and against atherosclerosis.OBJECTIVE：To observe the therapeutic effect of Bu Yang Huan Wu Tang on the treatment of atherosclerosis model,meanwhile,to investigate the mechanism on molecule level.METHODS：High fat diet and Vitamin D3 were used to induce a rat atherosclerosis model.After the AS rats model establishement,thirty-two rats model were divided into four groups according to random touch ball method：Bu Yang Huan Wu Tang therapy group,ligustrazine therapy group,model group and normal control group.The level of blood lipid and the expression of VCAM-1 mRNA in each group were observed.RESULTS AND CONCLUSION：The rate of molding success was 92%.The level of blood lipid in Bu Yang Huan Wu Tang therapy group was close to normal level,and there was no significant difference when compare with other two groups.There was no expression of VCAM-1 mRNA in normal control group,and compared with ligustrazine therapy group and model group,the expression of VCAM-1 mRNA in Bu Yang Huan Wu Tang therapy group was decreased significantly.It indicates that representative formula of qi-benifiting and blood-activating Chinese medicinal herbs Bu Yang Huan Wu Tang can down-regulate the expression of VCAM-1 mRNA,and decrease the level of blood-lipid in rat models.It has the effect against atherosclerosis,and the mechanism may be related with the down-regulated expression of VCAM-1mRNA.
出处
《中国组织工程研究》
CAS
CSCD
2012年第15期2776-2779,共4页
Chinese Journal of Tissue Engineering Research
基金
山西省卫生厅科技攻关项目(200958)
课题名称:补阳还五汤对动脉粥样硬化模型主动脉血管细胞黏附分子1mRNA表达量的影响~~
