摘要
目的探讨肺结核组织B细胞中Oct-2、Bob.1的表达与机体免疫的关系。方法采用免疫组织化学检测20例肺结核组织B细胞中Oct-2、Bob.1的表达情况。结果 (1)在肺结核组织生发中心的B细胞中Oct-2和Bob.1主要表达在细胞核;在无形成生发中心聚集的B细胞中,细胞核和细胞浆均呈阳性;(2)肺结核组织B细胞中Oct-2阳性的细胞数明显少于Bob.1阳性的细胞数,Oct-2和Bob.1阳性率分别为:10.8%、65.6%;(3)形成生发中心的肺结核组织B细胞中Oct-2和Bob.1阳性的细胞数明显高于无生发中心形成的肺结核组织,差异有统计学意义(P<0.05)。结论肺结核组织B细胞Oct-2表达低下和/或Oct-2、Bob.1表达不一致可能导致B细胞在发育过程中未能形成生发中心。
Objective To study the relationship between expression level of Oct-2 and Bob.1 in patient with tuberculosis and their immune system and pathological variation.Methods Expression profile of Oct-2 and Bob.1 were stained in lung tissues from 20 cases of tuberculosis by immunohistochemistry.Results（1） Oct-2 and Bob.1 were mainly expressed in B cells,nucleus of germinal center in patient with tuberculosis,but both markers were expressed in B cells;nucleus and plasma formation without the germinal center.（2） The positive numbers of Oct-2 were evidently less than that Bob.1 in lung tissues with tuberculosis and their positive rate were 10.8% and 65.6% respectively.（3） In lung tissues with tuberculosis,the positive numbers of Oct-2 and Bob.1 in formation of germinal center were significantly higher than without germinal center（P0.05）.Conclusions In B cells of lung tissues with tuberculosis,the lower expression of Oct-2 and/or Oct-2 and Bob.1 expression different may be the cause that B cell cannot form germinal center.
出处
《临床肺科杂志》
2012年第6期1079-1081,共3页
Journal of Clinical Pulmonary Medicine
