摘要
目的研究阿托伐他汀对大鼠造影剂肾病（CIN）有无保护作用及其给药时间与保护作用之间的关系。方法SD大鼠48只随机分为A（空白对照组）、B（实验对照组）、C（造模组）、D（阿托伐他汀1组）、E（阿托伐他汀2组）、F（阿托伐他汀3组）6组（每组n=6），建立CIN大鼠模型，并按相应要求分别予同一剂量的不同给药时间进行阿托伐他汀灌胃。全自动生化分析仪检测造模前后血肌酐（Scr）；光镜下肾组织病理观察并进行肾小管损伤半定量评分。结果①注射造影剂48h、72h后C组Scr较注射前均升高大于25％（P〈0．05），提示造模成功；②A组与B组相应时间点Scr差异无统计学意义（P〉0．05）；③与C组比较，D、E、F组注射造影剂48h、72h后血Scr有所下降（P〈0．05）；④注射造影剂前3天至后3天连续给药的F组与注射造影剂当天给药的D组、注射造影剂前3天给药的E组相比较，及注射造影剂前3天给药的E组与注射造影剂当天给药的D组，注射造影剂后48h、72h血Scr均有下降（P〈0．05）；⑧C组。肾小管损伤评分明显高于A组和B组（P〈0．05）；D、E、F组肾小管损伤评分低于c组（P〈0．05）；与D组相比，E、F组肾小管损伤评分有所降低（P〈0．05）；与E组相比，F组肾小管损伤评分有所降低（P〈0．05）；⑥给药时间与肾小管损伤评分呈负相关（r=-0．555，P〈0．01）。结论阿托伐他汀对大鼠造影剂肾病有保护作用，且注射造影剂前后3天连续给药较前3天给药及当天给药保护效果更加明显。
Objective To investigate the protective effect of atorvastatin on contrast - induced nephropathy （CIN） in rats, and the relationship between the different time of administering atorvastatin and the protective effect. Methods 48 Sprague - Dwaley rats were randomly divided into 6 groups （ n = 8 each） ： blank control group （ group A）, experimen- tal control group （group B）, CIN model -building group （group C）, atorvastatin treated group 1 （group D）, atorvasta- tin treated group 2 （group E）, and atorvastatin treated group 3 （ group F）. The rat model of CIN was established and atorvastatin was given in the same dose administered intragastrically but at different time for different groups with CIN. Serum creatinine （Scr） levels were determined by automatic biochemistry analyser. The pathologic changes of the kidney were observed by light microscopy and renal tubular injury was evaluated semiquantitatively. Results （1）48 h and 72 h after contrast agent injection, Scr level in group C increased over 25% than before contrast agent injection, indicating that CIN model was successfully established. （2）No statistically significant difference of Scr levels was observed between group A and B at the same time point （P 〉 0.05）. （3）Compared with group C, the Scr levels in group D, E, and F decreased at 48 h and 72 h after the injection of contrast agent （P 〈0. 05 ）. （4）Scr levels at 48 h and 72 h after contrast agent injection in group F （ atorvastatin was administered from 3 days before to 3 days after contrast agent injection） were lower than in group D （ atorvastatin was administered at the same day of contrast agent injection） and group E （ atorvastatin was ad- ministered for 3 consecutive days before contrast agent injection） （P 〈 0. 05）. Scr levels at the same time point in group E were lower than in group D. （5） Renal tubular injury scores in group C were obviously higher than in group A and B （P 〈 0.05） ; those in group D, E, and F were lower than in group C （ P 〈 0. 05） ; those in group E and F were lower than in group D （ P 〈 0.05 ） ; those in group F were lower than in group E （ P 〈 0.05 ） .（6） There was a negative correlation between different time of administering atorvastatin and the renal tubular injury scores （ r = - 0. 553, P 〈 0.01 ）. Conclusion Atorvastatin has the protective effect on CIN in rats. The protective effect is more obvious when atorvastatin is administered consecutively from 3 days before to 3 days after contrast agent injection compared to administered at the same day of contrast agent injection or administered for 3 consecutive days before contrast agent injection.
出处
《徐州医学院学报》
CAS
2012年第4期239-242,共4页
Acta Academiae Medicinae Xuzhou
关键词
阿托伐他汀
造影剂
肾病
大鼠
atorvastatin
contrast - media nephropathy
rat
