摘要
目的:探讨双丁酰环腺苷酸(dbcAMP)对甲状腺鳞癌SW579细胞株增殖的影响。方法:将dbcAMP0.5、1.0、2.0mmol/L作用于甲状腺鳞癌SW579细胞24h后,用四甲基偶氮唑蓝比色(MTT)法检测SW579细胞增殖的变化;经dbcAMP0.5mmol/L作用24h后,ELISA法检测细胞成熟促进因子(MPF)的活性,WesternBlot方法检测cdc2-Tyr15磷酸化水平,流式细胞术检测细胞周期变化情况。结果:与对照组相比,经不同浓度dbcAMP处理的SW579细胞株的增殖均受抑制。在0.5mmol/LdbcAMP作用下24h,SW579细胞MPF活性为(22.87±2.57)ng/L,远小于对照组的(115.50±7.53)ng/L,差异有统计学意义(t=3.324,P<0.05)。实验组cdc2-pTyr15磷酸化水平(0.258±0.026)高于对照组的(0.142±0.009),差异有统计学意义(t=7.424,P<0.05)。与对照组相比,应用0.5mmol/LdbcAMP处理细胞之后,G0/G1期无显著变化,S期细胞数量减少,G2/M显著增多。结论:dbcAMP能够显著降低甲状腺鳞癌SW579细胞株的活力,使细胞增殖受到明显抑制。
Objective： To study the effect of dual dibutyryl cyclic AMP（dbcAMP） on the proliferation of SW579 cell line. Methods ： The growth activity of cells were detected by MTT, after SW579 cells were treated with dbcAMP （0.5, 1.0, 2.0 mmol/L） for 24 h. At the level of 0.5 mmol/L, the activity of maturation promoting factor （MPF） were tested through ELISA. The protein expression of the phosphorylation level of cdc2-Tyrl5 was measured by Western blot. Changes of the cell cycle were de- tected by flow cytometry. Results： Compared with control group, the growth activity of SW579 cells was reduced after treatment with different levels of dbcAMP. Results of ELISA showed that the activity of MPF was （22.87±2.57） ng/L after treatment with 0.5 mmol/L dbcAMP for 24 h, which was significantly lower than that of control group （115.5±7.53） g/L （t = 3.324, P 〈 0.05）. The protein express of the phosphorylation level of ede2-Tyrl5 was higher in test group （0.258±0.026） than that of control group （0.142±0.009, t = 7.424 ,P 〈 0.05）. Compared with control group, the number of SW579 cells had no significant difference in GO/ G1 phase reduced in the S phase and increased obviously in the G2/M phase after treating with 0.5 mmol/L dbcAMP. Conclu- sion： dbcAMP could significantly reduce the vitality of SW579 cells, by which the cell proliferation was significantly inhibited.
出处
《天津医药》
CAS
北大核心
2012年第6期537-539,共3页
Tianjin Medical Journal
基金
辽宁省教育厅重点实验室项目(项目编号:LS2010101)
