摘要
目的研究let-7b对人黑色素瘤细胞增殖及有氧糖酵解的影响。方法将人工合成的microRNA单核苷酸let-7bmimics及inhibitor通过脂质体转染人黑色素瘤细胞株A375，上调或抑制let-7b在细胞内的表达水平，不同时相点检测细胞葡萄糖消耗和乳酸产量，MTT法检测细胞增殖。运用单因素方差分析探讨let-7b表达水平与黑色素瘤细胞有氧糖酵解及增殖的关系。结果let-7bmimics组24、48h时相点检测MTr吸光度值分别为0．396±0．030、0．525±0．037，低于其他组（P〈0．05），提示高表达let-7b抑制细胞增殖；各组黑色素瘤细胞24、48h葡萄糖消耗及乳酸产量差异无统计学意义（P〉0．05），乳酸产量与葡萄糖消耗比值各组间差异无统计学意义（P〉0．05），其中空白组在24h和48h内葡萄糖转化为乳酸效率约为57％和43％。结论黑色素瘤A375细胞具有显著的有氧糖酵解表型，过表达let-7b可抑制人恶性黑色素瘤细胞A375增殖，但对于细胞的有氧糖酵解效率，尚未发现let-7b对其有显著影响。
Objective To study the influence of let-7b on ceU proliferation and aerobic glycolysis of human melanoma cell A375. Methods Transfect A375 cell line with hsa-let-7b oligonucleotide or antisense. Glucose and lactate in medium were determined by spectrophotometry at 24 h and 48 h time point after trans- fection. The cell proliferation was determined by methylthiazol tetrazolium （MTF） assay. Results Over expression of let-Tb in melanoma cell reduced cell proliferation notably, compared to the other groups by MTT（ P 〈 0. 05）. However, the glucose consumption and lactate production differences were not observed during 24 h or48 h （ P 〉0. 05） , the blank control group transformed about 57% and 43% glucose to lactate during 24 h and 48 h. Conclusions Melanoma cell line A375 has notably aerobic glycolysis hallmark, let7b could inhibit proliferation of melanoma cell line A375, but it may has no influence on glucose metabolism.
出处
《中国医师杂志》
CAS
2012年第5期577-579,584,共4页
Journal of Chinese Physician
基金
国家自然科学基金（81071645,81171882）、中央高校基本科研业务费专项资金资助（2011JQ028）、湖南省自然科学基金（10JJ3030）、湖南省卫生厅科研基金（B2010031）和湘雅三医院“125”人才基金资助
