摘要
目的 :探讨小儿喉乳头状瘤 (JL P)人乳头瘤病毒 (HPV)感染途径及发病机理。方法 :采用 PCR及 PCR产物斑点杂交技术检测 JL P组织 HPV- DNA;散射免疫比浊法测定血清 Ig及补体 C3。结果 :JL P组织 HPV总感染率为 95 % (19/ 2 0 ) ,其中 HPV6 型为 5 5 % (11/ 2 0 ) ,HPV1 1 为 30 % (6 / 2 0 ) ,HPV6 + 1 1 型为 10 % (2 / 2 0 ) ;JL P患者血清 Ig G、Ig A、Ig M、C3值正常 ,较对照组无显著差异 (P>0 .0 5 )。结论 :小儿 HPV感染以 HPV6 型为主 ,和女性生殖道尖锐湿疣相一致。体液免疫在 HPV感染过程中影响较小。
Objective:To study HPV infection route and pathogenesis of juvenile laryngeal papilloma(JLP).Method: HPV DNA of JLP was detected with PCR and PCR product dot blot hybridization. Serum Ig and complement3(C 3) was detected with nephelometry. Result: HPV total positive rate in JLP was 95%(19/20). HPV 6 was 55%(11/20). HPV 11 was 30%(6/20), and HPV 6+11 was 10%(2/20). Serum IgG, IgA, IgM and C 3 of JLP were normal, no significant difference between JLP and the control group(P> 0.05 ). Conclusion: HPV 6 infection of JLP was in the majority. And the type was consistant with the type of female genital organ pointed condyloma. Humoral immumity was negligible in HPV infection. Pathologic picture of different HPV type infection was identical.
出处
《临床耳鼻咽喉科杂志》
CSCD
2000年第6期262-264,共3页
Journal of Clinical Otorhinolaryngology
基金
温州市科委科技发展基金!资助 (S99930 6 A)
关键词
喉肿瘤
乳头状瘤
乳头状瘤病毒
体液免疫
DNA
Child Laryngeal neoplasm Papillome Human papilloma virus Humoral immunity DNA Viral
