摘要
以新陆早33号为对照研究新疆主栽品种新陆早42号的体细胞胚胎发生。研究表明,所用4种激素组合均能有效诱导愈伤组织,但二者仅在经过IBA 1.0 mg.L-1+KT 0.5 mg.L-1和2,4-D 0.1 mg.L-1+KT 0.1 mg.L-1两种激素组合诱导初生愈伤后,才能胚胎发生。新陆早42号在IBA 1.0 mg.L-1+KT 0.5 mg.L-1培养基上46 d就开始胚胎发生;新陆早33号则不能直接胚胎发生,需继代到MSBP培养基上培养48 d才有胚胎发生。经2,4-D 0.1 mg.L-1+KT 0.1 mg.L-1组合培养的愈伤均需要继代到MSBP培养基上培养才能胚胎发生,新陆早42号和新陆早33号胚胎发生的最早时间分别是71 d和81 d。胚性愈伤在铺有滤纸的MSBF培养基上分化成胚并发育成再生植株。新陆早42号在140 d有根系发育良好的能嫁接植株(株高7～8 cm),而新陆早33需180 d。即成功建立了新陆早42号的再生体系,且其胚胎发生能力和再生能力均优于对照。
Somatic embryogenesis of leading variety in Xinjiang Xinluzao42 was studied, and compared with another variety Xinluzao33. Calli were effectively produced on media with four different hormone regimes and embryonic calli were only induced on media with IBA 1.0 mg·L-1 ＋ KT 0.5 mg-L-~ or 2,4-D 0.1 mg·L-1 ＋ KT 0.1 mg·L-1 hormones. Embryonic calli of Xin-1uzao42 were effectively initiated in 46 d on medium with IBA 1.0 mg·L-1 ＋ KT 0.5 mg·L-1, otherwise Xinluzao33 did not unless whose calli were transferred to medium of MSBP for subculture 48 d. Embryonic calli appeared after 71 d and 81 d respectively in Xinluzao42 and Xinluzao 33. For both cultivars, callus produced on the medium with 2,4-D 0.1 mg·L-1 ＋ KT 0.1 mg·L-1 must be transferred to medium of MSBP for subculture. Embryonic callus in the MSBF medium covered with filter paper differentiated into embryo and then plants regenerated. It took about 140 d to obtain regenerated plants （height 7-8 cm） for somatic cell of Xinluzao42, but about 180 d for Xinluzao33. A successful regeneration system were established for Xinluzao42, embryogenic and regeneration ablity of whom is better than Xinluzao33.
出处
《棉花学报》
CSCD
北大核心
2012年第3期238-243,共6页
Cotton Science
基金
国家转基因专项(2011ZX005-004)
兵团博士资金项目(2009jc01)
关键词
棉花
新陆早42号
体细胞胚胎
植株再生
Gossypium hirsumm L.
Xinluzao 42
somatic embryogenesis
regeneration
