摘要
目的建立通腑颗粒的质量标准。方法采用薄层色谱法对方中的大黄、枳实、厚朴进行定性鉴别,用高效液相色谱法对芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行含量测定。结果薄层色谱斑点清晰,分离度良好,阴性对照无干扰。芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.038～0.610、0.048～0.780、0.068～1.076、0.056～0.904、0.022～0.336μg范围内与峰面积积分值呈良好的线性关系,平均回收率依次为96.01%、94.67%、100.94%、90.06%、92.37%,RSD分别为1.90%、2.77%、2.18%、1.88%、2.84%。结论定性定量方法简便、结果准确可靠,所建立的标准可用于通腑颗粒的质量控制。
Objective To establish the quality standard of Tongfu granule. Methods TLC was used for the qualitative identification of Rheum palmatum L., Citrus aurantium L. and Magnolia officinalis. HPLC was used to determine the content of aloe-emodin, rhein, emodin, chrysophanol and physcion. Results TLC spots were clear and weU-separated without negative interference. The linear range of aloe-emodin, rhein, emodin, chrysophanol and physcion was 0.038--0.610, 0.048--0.780, 0.068--1.076, 0.056--0.904 and 0.022--0.336μg, respectively. The average recovery was 96.01%, 94.67%, 100.94%, 90.06% and 92.37%, respectively. RSD was 1.90%, 2.77%, 2.18%, 1.88% and 2.84%, respectively. Conclusion The method is simple, accurate and reliable with good precision. It can be used for the quality control of Tongfu granule.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第6期57-59,共3页
Chinese Journal of Information on Traditional Chinese Medicine
