摘要
目的:观察半夏泻心汤对伊立替康(irinotecan,CPT-11)致小细胞肺癌(small-cell lung cancer,SCLC)荷瘤鼠胃损伤是否具有改善作用。方法:建立SCLC荷瘤鼠,将40只荷瘤鼠随机分成4组,高、中、低3个给药组半夏泻心汤剂量分别为20、10、5g.kg-1.d-1,空白组给等量水,灌胃给药,共8天。第3天开始尾静脉注射CPT–11,35 mg.kg-1.d-1,共4天。观察荷瘤鼠食量情况;第8天处死荷瘤鼠,取胃组织,显微镜下观察其病理形态改变,免疫组化测胃黏膜环氧化酶-2(Cyclooxygenase-2,COX-2)表达;另取胃组织,行组织匀浆,离心,酶联免疫吸附法(Enzyme-linked immunosorbent assay,EL ISA)检测胃组织中血栓烷B2(TXB2)和6-酮-前列腺素F1α(6-keta-PGF1α)含量;取舌黏膜组织,显微镜下观察其病理形态改变。结果:半夏泻心汤给药组相较于空白组食量增加,空白组胃黏膜内及黏膜下少量血管有扩张,稍有水肿表现,舌黏膜空白组表皮完整,少量细胞空泡变性,鳞状上皮增生,表面角质层增厚,给药组均好于空白组。胃组织中TXB2和6-keta-PGF1α含量及胃黏膜COX-2表达情况各组无统计学差异。结论:半夏泻心汤对CPT-11致SCLC荷瘤鼠胃损伤具有一定的改善作用。
Objective：To observe the improvement of Banxia Xiexin Decoction against irinotecan-induced（CPT-11）spleen deficiency of tumor-bearing mice with small-cell lung cancer（SCLC）.Methods：Banxia Xiexin Decoction was administered to mice for eight days.CPT-11 was given from the 3rd day to the 6th day.The activity,weight and diarrhea of each mouse were recorded.Enzyme-linked immunosorbent assay（ELISA）was used to analyze the concentrations of gastrin in blood which was obtained from eyeballs at the 8th day.Results：The activity of the blank group was weaker than that of other three groups.The body weight increase both in the high and low dose group was more than that of the blank group from the 1st day to the 8th day.The diarrhea in the administrated groups was better than the that in the blank group.There were no significant differences of the gastrin concentrations between each administrated group and the blank group.Conclusion：Banxia Xiexin Decoction could improve the spleen deficiency induced by CPT-11 in nude mice bearing the tumor of SCLC.
出处
《中华中医药学刊》
CAS
2012年第6期1289-1291,I0012,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划项目(2010ZB006)
关键词
半夏泻心汤
胃损伤
伊立替康
Banxia Xiexin Decoction
injury of stomach
Irinotecan
