摘要
目的研究脑缺血/再灌注损伤后诱导细胞死亡DNA片段因子-45样因子-B(CIDE-B)表达在神经元凋亡的作用。方法成年健康雄性Wistar大鼠24只,随机分为假手术组(n=6)和脑缺血30min(n=6)、90min(n=6)和120min(n=6)再灌注组。参照Pusinelli四动脉阻断法建立大鼠全脑缺血再灌注模型。采用TUNEL法染色观察海马区神经元凋亡,免疫组织化学检测CIDE-B表达。结果大鼠脑缺血30min、90min和120min,海马区凋亡神经元数量较假手术组均显著增加(t=3.12,P<0.05;t=3.94,P<0.01;t=2.47,P<0.05);同时,CIDE-B阳性神经元数量较假手术组均有明显增加(t=2.46,P<0.05;t=3.93,P<0.01;t=2.28,P<0.05)。结论脑缺血/再灌注损伤后CIDE-B表达与神经元凋亡呈时相依赖性。
Objective To explore the effect of the expression of cell-death-inducing DFF45-like effector-B （CIDE-B） in neuron apoptosis after cerebral ischemia-reperfusion injury. Methods 24 adult healthy male Wistar rats were randomly divided into sham operation group and cerebral ischemia 30 min , 90 min and 120 min groups averagely. Rat models with global cerebral ischemia-reperfusion were produced by 4-VO method. TUNEL assay was used to observe the neuron apoptosis and the immunohistochemical assay was used to determine the expression of CIDE-B in hippocampus. Results Compared with sham operation group, the number of neuron apoptosis increased significantly in those after ischemia 30 min, 90 min, 120 min （t = 3.12, P 0.05; t = 3.94, P 0.01; t = 2.47, P 0.05）. Simultaneously, the expression of CIDE-B positive neurons increased significantly than those in sham operation groups （t = 2.46, P 0.05; t = 3.93, P 0.01; t = 2.28, P 0.05）. Conclusions The expression of CIDE-B is time-dependent on neurons apoptosis after cerebral ischemia-reperfusion injury.
出处
《临床医学工程》
2012年第6期884-885,共2页
Clinical Medicine & Engineering
