摘要
目的 研究枯否细胞抑制剂氯化钆在大鼠肝脏冷保存时对枯否细胞 (kupffercell,以下简称KC)的影响。方法 将Wistar大鼠随机分成 8组 ,每组 5只。A、B、C、D组为对照组 ,肝脏保存时间分别为 1、2、3、4h ,其余 4组为相应的实验组 ,各组大鼠经手术灌注、取肝后于 0～ 4℃生理盐水中保存。测定各组动物肝组织脂质过氧化物 (LPO)含量 ,做组织学和超微结构的观察。结果 随着保存时间的延长 ,KC呈激活状态 ,肝组织线粒体脂质过氧化程度增加 ,肝细胞尤其是肝细胞线粒体损伤逐渐加重。与对照组比较 ,实验组预用氯化钆后 ,肝脏组织学及超微结构病理损伤程度较轻。肝组织LPO水平较低 (P <0 0 5 )。结论 KC的激活与冷保存肝脏的结构和功能的变化关系密切。氯化钆可以抑制KC的激活 ,对肝脏冷保存损伤有一定的保护作用。
Objective [WT5”BZ] To investigate the effects of gadolinium chloride hexahydrate (GDCL 3 ) on kuppfer cell (KC) of rat liver in cold storage.[WT5”HZ] Method [WT5”BZ] Forty rats were randomized into 8 groups(5 in each group). As a control, the livers in group 1,2,3 and 4 were preserved 1?h,2?h,3?h and 4?h in cold saline solution respectively.The group 5 through 8 served as experimental group treated by GDCL 3 instead (7?mg/kg).LPO level was measured, optical and electron micrscope observation were performed. [WT5”HZ]Results [WT5”BZ] Ultrastructural examination showed abnormaly activated KC and injury of hepatocellular mitochondria along with the prolongation of cold storage. While treated by GDCL 3), the level of LPO in hepatic tissue was lower (compared with group 1 and 2, P <0 05) and the injury of liver cell mitochondria was less severe.[WT5”HZ] Conclusion [WT5”BZ] Our findings suggest that there is a close relation between activation of KC and the function and construction of the liver in cold storage.GDCL 3 inhibits the activation of KC and protects the function and ultrastructure of the liver cell in cold preservation. [WT5”HZ]
出处
《中华普通外科杂志》
CSCD
2000年第4期219-222,共4页
Chinese Journal of General Surgery
基金
辽宁省自然科学基金!课题资助项目 ( 6190 2 5 )
