摘要
目的探讨低氧诱导因子-1α(Hgpoxia-inducible factor-1α,HIF-1α)、锌指转录抑制因子Twist和钙黏蛋白E(E-cadherin)在人乳腺癌中的表达及其意义。方法采用免疫组化SP法检测术前未接受过放疗、化疗及激素治疗的69名患者乳腺癌组织标本中HIF-1α、Twist和E-cadherin的表达,并分析三者表达的相关性。结果在69份乳腺癌组织标本中,HIF-1α、Twist和E-cadherin的阳性表达率分别为71.01%(49/69)、57.97%(40/69)和50.72%(35/69);HIF-1α、Twist和E-cadherin的阳性表达率与TNM分期和腋淋巴结转移有关(P<0.05或P<0.01),与患者年龄、肿瘤大小和病理类型无关(P>0.05)。HIF-1α与Twist的表达呈显著正相关(r=0.286,P<0.05),Twist与E-cadherin的表达呈显著负相关(r=-0.371,P<0.01)。结论 HIF-1α、Twist和E-cadherin在乳腺癌中的表达情况可作为监测乳腺癌发展的指标。
Objective To investigate the expressions of hypoxia-inducible factor-1α （HIF-1α）, Twist （a zinc finger tran- scription inhibitor） and E-cadherin in human breast cancer tissue and their significance. Methods The expressions of HIF-1α, Twist and E-cadherin in breast cancer tissue specimens from 69 patients receiving no radiotherapy, chemotherapy or hormone therapy before surgery were determined by immunohistochemical assay with SP staining, between which the relationship was analyzed. Results The positive rates of HIF-1α, Twist and E-cadherin in the breast cancer tissue specimens were 71.01%（49/69）, 57. 97%（40/69） and 50. 72%（35 / 69） respectively, which were related to the TNM stage and axillary lymph node metastasis （P 〈 0. 05 or P 〈 0. 01 ）, but unrelated to the age of patient as well as size and pathological type of tumor （P 〉 0. 05）. The expression of HIF-1α was positively related to that of Twist （r = 0.. 286,P 〈 0. 05）. However, the expression of Twist was negatively related to that of E-cadherin （r = -0. 371 ,P 〈 0. 01）. Conclusion The expressions of HIF-1α, Twist and E-cadherin might be used as markers for monitoring the progress of breast cancer.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第7期884-887,共4页
Chinese Journal of Biologicals
基金
国家自然科学基金面上项目(81072183)
重庆市自然科学基金重点项目(CSTC
2011BA5038)
重庆医科大学重点科研课题(XBZD201002)
