摘要
目的明确梗阻性无精子症（OA）患者附睾精子核成熟度情况。方法选取2011年1—5月上海交通大学医学院附属仁济医院泌尿男科门诊的男性不育患者81例，其中拟行卵胞质内单精子注射技术（ICSI）的OA患者32例（OA组），弱精子症患者49例（弱精症组）；另设对照组32例。弱精症组及对照组进行精液分析，OA组进行经皮附睾精子抽吸术后进行样本镜检。同时采用吖啶橙试验及苯胺蓝染色试验对3组样本进行精子核成熟度检测。结果弱精症组与对照组的精液量及精子密度差异均无统计学意义（均P〉0．05），OA组精子密度显著低于对照组（P〈0．01）；弱精症组及OA组精子活率显著低于对照组（均P〈0．01）；而弱精症组a＋b级精子百分比也显著低于对照组（P〈0．01）。OA组和弱精症组的吖啶橙染色与苯胺蓝染色阴性率分别为57％±20％、64％±20％和62％±19％、67％±15％，均低于对照组（81％±9％、85％±8％），差异均有统计学意义（均P〈0.05）。结论OA患者附睾精子核成熟度低于健康对照，需要进一步改善精子核成熟度以提高生育力。
Objective To ascertain the epididymal sperm nuclear maturity in obstructive azoospermia （OA） patients. Methods A total of 81 infertile males from andriatry clinic of Renji hospital were selected, including 32 OA patients （ OA group） and 49 asthenospermia patients （ asthenospermia group）. Another 32 fertile males were recruited for the control group. All semen samples of the asthenospermia and control groups underwent computer aided semen analysis （CASA）. For the OA group, sperm specimens were collected by percutaneous epididymal sperm aspiration （PESA） and semen analyses completed by manual method. Further acridine orange test and aniline blue stain assay were performed. The results were processed with SPSS 15.0. Results No statistically significant differences existed in semen volume and sperm density between the asthenospermia and control groups （ both P 〉 0. 05 ）. The OA group was significantly lower than the control group in sperm density （ P 〈 0. 01 ）. The asthenospermia and OA groups were also significantly lower than the control group in sperm motility （ both P 〈 0. 01 ）. As to the percentage of grade a ± b sperm, the asthenospermia group was also significantly lower than the control group （P 〈 0. 01）. Compared with the control group （81% ± 9%, 85% ± 8% ）, the negative rate of acridine orange test and aniline blue assay of OA （57% ±20%, 64% ±0% ） and asthenospermia group （62% ± 19%, 67% ±15% ） were statistically lower （ all P 〈 0.05 ）. Conclusions The sperm nuclear maturity is lower in the asthenospermia and OA groups than that in the control group. Male fertility should be further improved
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第26期1828-1831,共4页
National Medical Journal of China
基金
上海市人口和计划生育委员会科研基金（2010JG03）
关键词
无精子症
精子核成熟度
经皮附睾精子抽吸术
吖啶橙试验
苯胺蓝染色
Azoospermia
Sperm nuclear maturity
Percutaneous epididymal sperm aspiration
Acridine orange test
Aniline blue stain
