摘要
以黑木耳菌糠为原料,通过单因素试验和正交试验,确定超声波法浸提纤维素酶和木聚糖酶的最佳条件为:振幅90%、超声时间15 min、液料比为60 1。对比试验中,超声波法浸提液羧甲基纤维素酶活和木聚糖酶活为7.500和4.921 IU.g-1,高于水浸提法(对照)。粗酶液羧甲基纤维素酶活最适pH为4.4,最适温度60℃;滤纸酶活最适pH为5.2,最适温度60℃;木聚糖酶最适pH为4.8,最适温度为50℃。
Singlefactor and orthogonal experiments were employed to optimize the extracting conditions of crude cellulase and xylanase from residue of Auricularia auricular by ultrosonic wave method. Results showed that the optimal conditions were as follows： ultrosonic wave amplitude was 90%, processing time was 15 min, liquid-material ratio was 60： 1. In comparative experiments on extracting crude cellulase and xylanase, CMCase activity and xylanase activity of extract applied ultrosonic wave method were 7 500 and 4 921 IU .g-l, and were superior to water extraction method. The optimum pH and temperature of CMCase activity, filter paper activity were 4.4 and 60 ℃, 5.2 and 60℃ and xylanase activity 4.8 and 50℃, respectively.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第5期117-121,共5页
Journal of Northeast Agricultural University
基金
国家科技部科技人员服务企业行动资助项目(2009GJB20022)
关键词
超声波
纤维素酶
木聚糖酶
黑木耳
菌糠
酶学性质
ultrosonic wave
cellulase
xylanase
Auricularia auricular, residue
enzymological properties
