摘要
目的通过检测心肌组织特异性microRNA－1（miR－1）在sT段抬高性急性心肌梗死（acute myocardial infarction，AMI）患者血浆中的含量，探讨血浆miR-1在AMI诊断中的作用。方法20例AMI患者在发病12h内取血，20例健康志愿者血作为对照，用TaqMan实时荧光定量RT—PCR（TaqManreal—timequantityRT—PCR，qRT—PCR）检测血中miR—1含量，并同时检测血清肌钙蛋白I（cTnI）和肌酸激酶同工酶（CK—MB）。结果AMI患者血清cTnI、CK—MB和血浆miR-1含量明显高于正常值（P〈0．01），AMI患者血浆miR-1含量与血清CK—MB和cTnI含量呈直线正相关（P〈0．05）。结论血浆miR-1可作为AⅧ的-个新的敏感牛物学标志。
Objective To observe serum cardio - specific microRNA - 1 （ miR - 1 ） levels in ST segment elevation acute myocardial infarction patients, and to explore the effect of serum miR - 1 levels on the diagnosis of acute myocardial infarction （ AMI ）. Methods We assessed the serum concentrations of miR - 1 in 20 patients with AMI, and 20 healthy individuals as control. Serum miR - 1 concentrations were measured with a real - time reverse - transcription PCR （ qRT - PCR）, serum cardiac troponin I （cTnI） or creatine kinase - MB （ CK - MB） concentrations were measured. Results Serum level of cTnI, CK - MB and miR - 1 were obviously higher in AMI patients than in healthy individuals （P 〈 0.01 ） ; The serum miR - 1 level in the patients with AMI had a positive correlation with serum cTnI or CK - MB （P 〈 0.05 ）. Conclusion Serum miR - 1 could be a novel sensitive biomarker of AMI.
出处
《中国急救医学》
CAS
CSCD
北大核心
2012年第7期579-582,共4页
Chinese Journal of Critical Care Medicine
基金
四川省科技厅国际合作课题（No.2011HH0031）
