摘要
利用硅胶柱、凝胶柱及高效液相(HPLC)等色谱方法,对采自中国南海的深海芽孢杆菌E401B03发酵液的乙酸乙酯相浸膏进行了分离,通过1H-NMR、13C-NMR、EIMS等方法鉴定了7个化合物:N-苯乙基乙酰胺、3-(羟基乙酰基)-吲哚、3-(2-羟基乙基)-吲哚、环(缬-缬)二肽、尿嘧啶、胸腺嘧啶、对羟基苯甲醛。
Seven compounds, were isolated from the ethyl acetate extract of Bacillus sp. E401B03. Compounds 1-7 were obtained by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were identified by spectroscopic methods including 1H-NMR,13C-NMR, and EIMS.
出处
《海洋科学》
CAS
CSCD
北大核心
2012年第6期28-30,共3页
Marine Sciences
基金
农业部海洋与河口渔业资源及生态重点开放实验室开放课题(开-09-03)
江苏省自然科学基金项目(BK2011395)
关键词
深海
芽孢杆菌
次生代谢产物
deep-sea
Bacillus sp.
secondary metabolites
