摘要
目的 探讨bcl 2反义脱氧寡核苷酸 (ODN)及反义RNA对人宫颈癌体外治疗的效果。方法 应用反义技术进行人宫颈癌Hela细胞生长抑制及凋亡诱导。结果 Hela细胞中bcl 2mRNA及蛋白均呈高表达。bcl 2反义ODN可有效抑制Hela细胞生长及降低其内源性bcl 2蛋白的表达 ,同时诱导其凋亡 ;bcl 2反义ODN浓度为 10 μmol/L时抑制效果最明显 ,抑制作用在转染后 2 4h效果最明显 ,持续至 96h后逐渐减弱 ,细胞凋亡程度与ODN用量呈区域性正相关 ;重组逆转录病毒载体(pDOR AB)能有效将bcl 2反义RNA导入Hela细胞实现稳定整合 ,同时降低bcl 2mRNA及蛋白表达 ,抑制Hela细胞生长并诱导其凋亡。结论 应用反义技术于体外治疗宫颈癌有一定效果 ,bcl 2基因作为优选靶向基因具有可行性 ,为进一步于体内治疗研究提供了理论基础和实验依据。
Objective To study the therapeutic effect of bcl 2 antisense oligodeoxynucleotide(ODN)/RNA human cervical carcinoma in vitro. Methods To transfect bcl 2 antisense ODN/RNA into Hela cells with antisense technology and observe the growth and apoptotic modulation of Hela cells. Results The expression of bcl 2 protein and mRNA was higher in Hela cells; the bcl 2 specific antisense ODN significantly decreased bcl 2 protein expression and inhibit the growth of Hela cells. The strongest inhibitory effect was at 24 hours after transfection, the optimal concentration of antisense ODN was 10 μmol/L. The results indicated that the inhibitory effect of bcl 2 antisense ODN on cell growth and survival rate is dose dependent; the recombinant retroviral vector pDOR AB can transfer antisense bcl 2 gene into the genome of Hela cells, bcl 2 protein and mRNA expressions were significantly decreased after transfected,it demonstrated bcl 2 antisense RNA can significantly inhibit the growth of Hela cells and induce their apoptosis. Conclusion The results demonstrate the feasibility of bcl 2 gene as a target for antisense gene therapy of human cervical carcinoma and provide the theoretical foundation and experimental evidence for further study in vivo.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2000年第2期105-108,I002,共5页
Chinese Journal of Obstetrics and Gynecology
关键词
子宫颈肿瘤
BCL-2基因
反义
基因转染
Cervix neoplsms
Oligonucleotides, antisense
Proto oncogene proteins
Transfection
Genes, bcl 2
