摘要
目的 研制一种适合我国眼库使用的保持角膜活性的中期保存液。方法 配制新的角膜活性中期保存液 (DX液 ) ,按不同天数保存兔角膜植片 ,分别进行角膜内皮细胞活性率、酶组织化学联合染色及超微结构观察 ,并将在DX液中保存 2～ 11d的 37只人眼角膜植片应用于临床穿透性角膜移植手术中。结果 兔实验结果显示 ,DX液与Optisol液差异无显著性 (P >0 0 5 )。 37只人眼角膜植片术后 1周内全部透明 ,内皮细胞密度均值为 (2 2 0 4 5 6± 6 89 5 6 )个 /mm2 。结论 DX液作为我国眼库角膜活性中期保存液安全可靠。
Objective To develop intermediate term storage media suitable for Chinese eye banks Methods Corneal buttons of rabbits were stored in DX solution freshly prepared After storage for several days, the survival rate of the corneal endothelial cells was examined, enzymohistochemical staining and ultrastructural examinations were carried out for the buttons Thirty seven human corneas stored in DX solution for 3 11 days were used for corneal transplantation, clinically Results There was no significant difference between rabbit corneal buttons stored in DX sol and in Optisol in any indices studied 37 grafts were all transparent in 1 week after transplantation, and the mean endothelial cell density was (2 204 56±689 56) cells/mm 2 Conclusion As a safe and efficient intermediate term (about 1 week) storage medium, DX sol is appropriate for Chinese eye banks
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2000年第1期21-23,I002,共4页
Chinese Journal of Ophthalmology
关键词
角膜移植
穿透性
保存液
中期保存液
Cornea
Keratoplasty, penetrating
Storage media
Mid term
