摘要
目的 探讨角膜碱烧伤后眼局部的免疫反应机制。方法 在大鼠角膜上制作碱烧伤模型。在烧伤后的不同阶段 ,制备角膜、虹膜组织平片 ,采用标准的卵白素 生物素过氧化物酶复合物免疫组化 (avidin biotincomplex ,ABC)方法 ,观察眼局部T淋巴细胞亚群、巨噬细胞、树突细胞、主要组织相容性复合物 (majorhistocompabilitycomplex,MHC) Ⅱ类抗原阳性细胞的动态变化。结果 碱烧伤后早期 ,角膜及虹膜即有T淋巴细胞浸润 ,以CD+ 3 淋巴细胞为主 ,MHC Ⅱ类抗原阳性细胞也轻度增多。在角膜溶解穿孔阶段 ,T淋巴细胞浸润达到高峰 ,其中CD+ 4淋巴细胞明显多于CD+ 8淋巴细胞 ;巨噬细胞、树突细胞、MHC Ⅱ类抗原阳性细胞大量出现。上述细胞密集分布于角膜缘 ,其中淋巴细胞及MHC Ⅱ类抗原阳性细胞可见于角膜溶解处及中央区。在烧伤后期角膜溃疡已进入修复阶段 ,虹膜上依然可见多量各类阳性细胞。结论 免疫反应可能参与了角膜重度碱烧伤后的组织病变过程 ,在角膜溶解穿孔及眼内炎症的发生中起重要作用。
Objective To investigate the immune reaction of severe corneal alkali burns. Methods The models of severe corneal alkali burns were induced in rats. Immunohistochemistry was carried out on wholemounts of the cornea and iris at different time points after corneal burns to detect T lymphocytes and their subpopulation, macrophages, dentritic cells and MHC class Ⅱ positive cells. Results In the early stage of the burns, there were small increasing number of T lymphocytes and MHC class Ⅱ positive cells in the cornea and iris. At the period of corneal melting and perforation, a massive influx of lymphocytes (CD + 3 , CD + 4 , CD + 8 ), macrophages and dentritic cells was seen in the cornea adjacent to the limbus and iris. CD + 4 lymphocytes were much more than CD + 8 in number. T cells and MHC class Ⅱ positive cells were observed not only at the limbus but also in the melting area and corneal center. Morphological changes of these cells were also noted. In the restoration stage, a number of positive cells were still found in the iris. Conclusion Immunological mechanism is involved in the pathological changes of the damage induced by corneal alkali burns, especially in the pathogenesis of the corneal melting and perforation.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2000年第1期40-42,I004,共4页
Chinese Journal of Ophthalmology
基金
美国中华医学基金会资助!(93 594)
关键词
角膜碱烧伤
免疫研究
大鼠
Cornea
Burn, chemical
Models,immunology
Immunohistochemistry
Wholemount
