摘要
目的应用高效液相色谱法测定活血清脑丸中阿魏酸的含量。方法色谱柱:Agilent Zorbax SB C18柱(4.6 mm×150 mm,5μm);流动相:甲醇-1%冰醋酸溶液(30∶70);流速为1.0 ml/min;检测波长为321 nm;柱温为30℃;进样量为10μl。结果阿魏酸在2.01～40.16μg/ml范围内与其峰面积线性关系良好(r=0.9999),平均回收率(n=6)为101.09%,相对标准偏差为1.88%。活血清脑丸供试品(批号20090316、20100719、20101207)中阿魏酸含量分别为125.7、165.2、98.5μg/g,相对标准偏差分别为1.33%、1.92%、1.37%。结论高效液相色谱法用于活血清脑丸阿魏酸的含量测定操作简便、准确可靠,有助于加强该制剂的质量控制。
Objective To use high performance liquid chromatography method（HPLC） for determination about content of ferulic acid in activating blood circulated-clear brain pill.Methods The Agilent Zorbax SB C18 pole（4.6 mm×150 mm,5 μm） was used,the mobile phase consisted of methanol-1% acetic acid（30∶70）,the flow rate was 1.0 ml/min,the detection wavelength at 321 nm,the column temperature at 30℃,and the inject volume 10 μl.Results A good linear correlation of ferulic acid was observed within the range of 2.01-40.16 μg/ml（r=0.9999）.The average recovery was 101.09% with RSD 1.88%（n=6）.The contents of feulic acid in activating blood circulated-clear brain pill（batch 20090316,20100719,20101207） were 125.7,165.2 and 98.5 μg/g with RSD 1.33%,1.92% and 1.37%,respectively.Conclusion The method is simple and accurate and can be used for the determination and quality control of ferulic acid in activating blood circulated-clear brain pill.
出处
《解放军医药杂志》
CAS
2012年第7期29-31,共3页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
关键词
活血清脑丸
阿魏酸
色谱法
高效液相
Activating blood circulated-clear brain pill
Ferulic acid
Chromatography, high performance liquidchromatography
