摘要
采用超滤法和Sephadex G-25凝胶色谱法对绿豆蛋白的酶解产物进行了分离纯化,得到了分子量为3426和1272u的两种绿豆抗氧化活性肽T1和T2,T1的纯度为85.92%,T2的纯度为94.99%,T1和T2均含有16种相同的氨基酸,表现出较强的体外抗氧化能力,T1对羟自由基和DPPH自由基的清除率分别为69.14%和58.62%;T2对羟自由基和DPPH自由基的清除率分别为91.70%和74.68%。
Two kinds of antioxidant peptides（named antioxidant peptide T1 and antioxidant peptide T2 separately） were separated from mung bean hydrolysates produced by neutral protease with ultrafiltration and Sephadex G-25 gel chromatography. The molecular weight of antioxidant peptide T1 was 3426u and its purity was 85.92%. The molecular weight of antioxidant peptide -i-2 was 1272u and its purity was 94.99%. Both of these two kinds of antioxidant peptide which were composed of sixteen kinds of amino acids presented strong antioxidant capacity. The scavenging rates of peptide T1 against hydroxyl radical and DPPH radical were 69.14% and 58.62% separately and the scavenging rates of peptide T2 against these two kind radicals were 91.70% and 74.68% separately.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第14期153-156,共4页
Science and Technology of Food Industry
关键词
绿豆抗氧化肽
分离
活性
组成
antioxidant peptide of mung bean
separation
activity
composition
