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运用DNA测序技术 提高不常见菌的鉴定水平 被引量：1

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摘要 临床微生物室的一项重要任务是对感染性疾病进行病原学诊断，同时为临床提供正确的药敏结果。及时、准确的病原学诊断能够帮助临床选择合适的抗感染药物，降低病死率，缩短住院时间。但是目前常规病原学检测手段远不能满足临床需求，主要受限于以下因素：（1）常规培养和药敏试验至少需要48h，对于分枝杆菌、厌氧菌等，需要更长时间；（2）广谱抗菌药物的使用极大地降低了培养的阳性率；（3）许多苛养菌和不常见菌，体外常规培养困难；（4）基于生化谱型的自动化：鉴定系统或其他表型方法对于特殊或不典型谱型的菌或数据库无法覆盖的菌，常常无法准确鉴定到种。
作者 王辉
机构地区 北京大学人民医院检验科
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2012年第7期577-578,共2页 Chinese Journal of Laboratory Medicine
关键词 鉴定系统 DNA测序技术 常见菌 临床微生物室 病原学诊断 广谱抗菌药物 常规培养 感染性疾病
分类号 R446.5 [医药卫生—诊断学]
  • 相关文献

参考文献6

  • 1Woo PC, Lau SK, Teng JL, et al. Then and now:use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin Microbiol Infect, 2008,14:908-934.
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二级参考文献14

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共引文献27

同被引文献18

  • 1陈东科,孙长贵.实用临床微生物学检验与图谱.北京:人民卫生出版社,2011:425.
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二级引证文献16

中华检验医学杂志
2012年 第7期

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