摘要
为优化柳枝稷愈伤组织诱导培养条件,以柳枝稷成熟胚为外植体,通过正交设计,研究基因型、2,4-D、6-BA、基本培养基、碳源和pH等因素对柳枝稷成熟胚愈伤组织诱导的影响。结果表明,6种因素中,2,4-D对柳枝稷成熟胚愈伤组织诱导的胚性愈伤率影响最大,6-BA次之,再次为基本培养基,而基因型、碳源和pH对胚性愈伤率影响较小。柳枝稷成熟胚愈伤组织诱导的最佳培养基组合为2,4-D 5 mg/L,6-BA0.2mg/L,基本培养基B5或MS或N6以及基因型西稷3号。柳枝稷成熟胚愈伤组织诱导的胚性愈伤率与出愈率、愈伤组织鲜质量和干质量均呈显著正相关关系,出愈率与愈伤组织干质量和愈伤组织鲜质量无显著相关性。
This study is expected to optimize callus induction condition and to find effects of six factors on callus induction from Switchgrass mature embryo through a medium screening experiment, mature embryos were used as explants. Orthogonal-design was used in the experiment for the factors including genotype, concentration of 2,4-D and 6-BA, basal medium, carbon resource and pH value comprehensively. Result showed the most important factor was concentration of 2,4-D, then concentration of 6-BA, and the basal medium, while genotype, carbon resource and pH value had no remarkable effects on callus induction. This experiment showed the best combination of factors in callus induction from Switchgrass mature embryo is 2,4-D 5 mg/L, 6-BA 0.2 mg/L, basal medium B5, or MS, or NB, genotype Xiji3. Embryogenic calli induction rate had significant positive correlation to callus induction rate, fresh mass and dry mass of callus, and callus induction rate had no significant correlation with fresh mass or dry mass of callus.
出处
《西北农业学报》
CAS
CSCD
北大核心
2012年第6期140-145,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(30871571)
"948"计划项目(2011-G1-19)
关键词
柳枝稷
愈伤组织
正交设计
成熟胚
相关性
Switchgrass （Panicum virgaturn L. ）
Callus
Orthogonal-design
Mature embryo
Correlation
