摘要
目的:研究提取糯米藤多糖的最佳工艺条件。方法:以多糖提取率为考察指标,溶剂回流作为提取手段,利用单因素及正交试验确定影响提取的主要因素,然后使用回归正交组合设计优化提取工艺。结果:影响糯米藤多糖提取率的因素顺序为提取温度>液固比>提取时间>提取次数>浸泡时间;回流提取的最佳工艺条件为在96.26℃水浴条件下,提取时间109.20min、提取次数2次、液固比27.28:1(mL/g)、浸泡时间90min,最优工艺条件下多糖提取率可达25.121%。结论:使用回归正交设计能合理地优化糯米藤多糖的提取工艺。
Objective： To find optimal conditions for the extraction of polysaccharides from whole plants of Gonostegia hirta （BL;） Miq. Methods： Solvent reflux extraction was used for polysaccharide extraction. Five parameters that influence extraction efficiency were investigated using one-factor-at-a-time and orthogonal array design methods, and three main ones were opti- mized based on a quadratic rotation-orthogonal composite experimental design. Results： In decreasing order of their effect on extraction efficiency, five influential parameters were ranked as extraction temperature 〉 solvent-to-solid ratio 〉 extraction time 〉 extraction number 〉 soaking time. Their optimal levels were 96.26 ℃, 27.28： 1 （mL/g）, 109.20 min each time, 2 and 90 min, respectively, resulting in an extraction yield of 25.121%. Conclusion： Quadratic rotation-orthogonal composite experimen- tal design permits reasonable optimization of polysaccharide extraction from Gonostegia hirta （BL.） Miq.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第14期50-54,共5页
Food Science
基金
西南民族大学2012年研究生学位点建设项目(2012XWD-S0703)
关键词
糯米藤
多糖
正交回归
提取率
Gonostegia hirta （BL.） Miq.
polysaccharides
quadratic rotation,orthogonal composite experimental design
extraction rate
