摘要
研究了半夏[Pinellia ternata(Thunb.)Breit]生物碱的诱导物硝酸镧、前体L-苯丙氨酸对其愈伤组织生长速率和生物碱含量的影响。结果表明,培养38天后,硝酸镧、L-苯丙氨酸对半夏愈伤组织生长速率及生物碱积累均没有显著影响。不同浓度处理中,2.25 mg/L硝酸镧和6.00 mg/L L-苯丙氨酸的生物碱积累量最高,分别比对照高76.89%和8.99%。随着添加浓度的增加,半夏愈伤组织生长速率和生物碱积累量总体呈先升后降的趋势。
The effects of lanthanum nitrate as elicitor and L - phenylalanine as precursor on callus growth rate and alkaloid content of PineUia ternata were studied in this paper. After cultured for 38 days, the results showed that they had no significant effects on the callus growth rate and total alkaloid content of Pinellia ternata. The content of alkaloid was the most when the concentration of lanthanum nitrate and L - phenylalanine were 2.25 mg/L and 6.00 rng/L respectively, which increased by 76.89% and 8.99% respectively compared with the check. With the increase of the added lanthanum nitrate and L - phenylalanine, the callus growth rate and alkaloid accumulation showed the tread of increasing firstly and then decreasing.
出处
《山东农业科学》
2012年第7期26-28,共3页
Shandong Agricultural Sciences
基金
山东农业大学大学生科研训练计划(SRT)资助(1112013)
关键词
半夏
硝酸镧
L-苯丙氨酸
愈伤组织
生物碱
Pinellia ternata
Lanthanum nitrate
L - phenylalanine
Tissue culture
Alkaloid
