摘要
目的:运用高效液相色谱法,对不同地区中麻黄生物碱(包括盐酸麻黄碱和盐酸伪麻黄碱)含量进行比较分析。方法:采用高效液相色谱法,色谱柱为C18(250 mm×4.6 mm,50μm],流动相为V[乙腈]:V[0.02%磷酸水溶液]=4:96,流速1.0 mL/min,柱温30℃,检测波长210 nm。结果:四产地样品麻黄碱含量和伪麻黄碱含量有显著性差异(P<0.05),麻黄碱含量新疆>青海>甘肃>山西,伪麻黄碱含量新疆>甘肃>青海>山西,但其总含量无显著性差异,均符合药典总碱含量不少于0.8%的用药标准。结论:通过对不同产地麻黄进行生物碱含量分析,为麻黄药材的利用提供依据,有助于麻黄药材的标准化种植及质量控制。
Objective： To make comparative analysis on contents of ephedra alkaloid （including ephedrine and D-pseudephedrine） with HPLC. Method： HPLC was used, chromatographic column was C 18 （250 mm×4.6 mm, 50 μm）, the mobile phase was V（methyl cyanide）：V（0.2% ammonium dihydrogen phosphate solution）=4：96, flow rates was 1.0 mL/min, the temperature was 30℃, length of detection wave was 210nm. Result： The contents of ephedrine （P〈0.05） and D-pseudephedrine in four samples from different habitats showed notable differences （P 〈0.05）, contents of ephedrine ranked Xinjiang 〉 Qinghai 〉 Gansu 〉 Shanxi, contents of D-pseudephedrine ranked X inj iang 〉 Gans u 〉 Qing- hal 〉 Shanxi, but comparison of total contents suggested no remarkable difference and all samples met the standard that content of total alkalis was not less than 0.8% in Pharmacopeia. Conclusion： Analysis on contents of alkaloid in MaHuang from different habitats could provide evidence for the utilization of MaHuang （Ephedrae）, helpful for stan- dardized plant and quality control of MaHuang.
出处
《西部中医药》
2012年第7期14-16,共3页
Western Journal of Traditional Chinese Medicine
基金
科技部十二五科技支撑项目(编号2011BAI05B02)
