摘要
采用MTT法对分离自美国圣璜岛海域的251株海洋细菌进行抗肿瘤活性的筛选,以期从海洋环境中筛选出具有抗肿瘤活性的菌株,为新型抗肿瘤药物开发提供新的天然产物资源。结果表明:54株海洋菌有较好的抗肿瘤活性,在测试浓度为100μg·mL-1时,对HeLa细胞的抑制率均大于50%,占总测试菌株的21.5%,其中有7株菌株对HeLa细胞的抑制率在85%以上。在所有活性菌株中,菌株No.657的活性最强,其液体发酵及固体发酵的提取物,对HeLa细胞的IC50分别为36.53、3.19μg·mL-1,经菌株形态的观察和16SrDNA序列分析鉴定,该菌株为变形斑沙雷氏菌Serratia proteamaculans。这表明,海洋微生物是抗肿瘤活性物质的重要来源,菌株No.657是一株很好的抗肿瘤活性菌,其固体发酵更适宜其活性代谢产物的产生。
The antitumor activity of some marine bacterial isolates was investigated to provide potential active strains as well as novel active compounds for the antitumor drug development.In this study,by using MTT assay,we screened for the antitumor activity from some marine bacterial strains which were isolated from San Juan Island.The results showed that,among 251 tested bacterial isolates,54 isolates（about 21.5%） showed better antitumor activity.Their inhibition rates against HeLa cell at the concentration of 100 μg·mL-1 were higher than 50%.And 7 of them showed stronger antitumor activity with the inhibitory rate higher than 85%.Among all the active strains,bacterium No.657 showed the most remarkable antitumor activities.The antitumor activities（IC50） of its metabolies extracted from liquid fermentation and solid-state fermentation were 36.53 and 3.19 μg·mL-1,respectively.This strains was identified as Serratia proteamaculans,according to its morphological observation and 16S rDNA sequence analysis.Our results indicated that marine microorganisms are an important source of antitumor substances.Isolate No.657 might be a good antitumor active strain and the solid-state fermentation should be better for its active metabolites production.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2012年第2期18-22,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(41076097
41006097
41106113)
新药研究国家重点实验室开放基金项目(SIMM1106KF-09)
关键词
海洋细菌
抗肿瘤活性
MTT法
抑制率
marine bacteria
antitumor activities
MTT assay
the inhibition rate
