摘要
采用连续根系分泌物收集系统(CRETS)收集大葱根系分泌物,研究其对黄瓜种子萌芽及其枯萎病病原菌的化感作用,并利用气相色谱—质谱技术(GC–MS)分析其化学组成。结果表明:大葱根系分泌物对黄瓜种子萌发和芽苗生长均表现低浓度促进、高浓度抑制的作用,‘元藏大葱’比‘章丘大葱’的化感作用更强;随着根系分泌物浓度升高,对病原菌菌丝生长和孢子萌发的抑制作用逐渐增强,‘元藏大葱’根系分泌物的抑制程度大于‘章丘大葱’。大葱根系分泌物中含有杂环化合物、酯类、酰胺类、酸类、烃类、醛酮类、酚类、醇醚类以及萘类等物质,其中,前三者所占的比例较大。邻苯二甲酸酯类和苯并噻唑类物质相对含量较高,2,2’–亚甲基双(4–甲基–6–叔丁基苯酚)、苯甲酸苄酯、苯甲酸–4–甲基苯酯、2–甲硫基异硫氰酸苯酯相对含量较低,同一物质‘元藏大葱’根系分泌物中相对含量高于‘章丘大葱’。
The allelopathy of welsh onion root exudates collected by continuous root exudates trapping system （CRETS） was studied on cucumber seed germination and Fusarium oxysporum f. sp. cucumerinum, and the components of the root exudates were identified by gas chromatography-mass spectrometry （GS - MS） . The results showed that the seed germination and sprout growth of cucumber were promoted by root exudates of welsh onion at low concentration while suppressed at high concentration, the allelopathy of ＇Yuanzang＇ was stronger than ＇Zhangqiu＇ . Inhibitory effects on hypha growth and spore germination of Fusarium oxysporum f. sp. cucumerinum enhanced with increasing concentration of root exudates, and ＇Yuanzang＇ showed stronger effects than ＇Zhangqiu＇ . Heterocyclic,ester, amide, acid, hydrocarbon, aldehyde, ketone, phenol, alcohol, ether, naphthalene compounds were detected in root exudates of Welsh onion, of which heterocyclic, ester and amide were the most compounds. Phthalate esters and benzothiazole derivatives had greater relative contents while p-cresol 2,2＇-methylenebis 6-tert-butyl-, benzyl benzoate, benzoic acid p-tolyl ester, 2- （methylthio） phenyl isothiocyanate had lower relative contents, which were higher in ＇Yuanzang＇ than in ＇Zhangqiu＇ .
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第8期1511-1520,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31071830)
高等学校博士学科点专项科研基金项目(20103702110005)
‘十二五’国家科技支撑计划项目(2011BAD12B04)
关键词
大葱
黄瓜
根系分泌物
化感作用
枯萎病病原菌
welsh onion
cucumber
root exudate
allelopathy
Fusarium oxysporum f. sp. cucumerinum
