摘要
目的采用高速逆流色谱法分离制备多花蒿中阿格拉宾。方法多花蒿乙醇提取物经硅胶柱色谱分离,所得组份Fr.1用高速逆流色谱进一步分离,采用正己烷-醋酸乙酯-甲醇-水(1.2:0.8:1.5:0.5)为两相溶剂系统,上相为固定相,下相为流动相,主机转速850r/min,体积流量2.0mL/min,检测波长为254nm。采用波谱法对所得化合物进行结构鉴定,HPLC法测定产品的纯度。结果从500mg组分Fr.1中得到260mg质量分数为99.5%的阿格拉宾。结论该方法操作简单,可用于阿格拉宾化学对照品的分离制备。
Objective To establish a method for the isolation and preparation of arglabin from Artemisia myriantha by high speed counter-current chromatography （HSCCC）. Methods The ethanol extract of A. myriantha was separated with silica gel column chromatography, and the obtained Fr. 1 was further separated by HSCCC with a two-phase solvent system composed of n-hexane-ethylacetate-methanol-water （1.2：0.8：1.5：0.5）. The lower phase was used as mobile phase, and the upper phase was used as the stationary phase. Main engine speed was 850 r/min, and the detection wavelength was 254 mn at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and analyzed by HPLC. Results A total of 260 mg arglabin with the purity of 99.5% was successfully obtained from 500 mg Ft. 1. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of arglabin reference substance.
出处
《现代药物与临床》
CAS
2012年第4期363-365,共3页
Drugs & Clinic
基金
科技部"十一五"重大新药创制项目(2010ZX09401-402)
关键词
多花蒿
阿格拉宾
高速逆流色谱
对照品
Artemisia myriantha Wall. ex Bess.
arglabin
high speed counter-current chromatography （HSCCC）
reference substance
