摘要
由于烟草的分子标记开发和遗传图谱构建十分困难,迄今烟草中有关数量性状基因座（QTL）的定位研究仍非常有限。本研究利用一个由207个株系组成的烤烟DH群体及基于该群体所构建的含有24个连锁群、611个SSR标记,总长为1882.1cM的遗传图谱,采用复合区间作图方法,对株高（PH）、茎围（SG）、节距（IL）、叶片数（LN）、最大腰叶长（LWL）和最大腰叶宽（WWL）6个与叶片产量有关的农艺性状进行QTL定位分析。共检测到69个QTL,大部分QTL的效应值较小,仅有4个具有较大的效应值,可解释大约15%~20%的表型变异。6个性状之间大多彼此相关。与此相符,在基因组中发现存在许多小区域,每个区域包含2个或2个以上紧密连锁的不同性状的QTL。
To identify quantitative trait lici （QTLs） for important traits in tobacco, a doubled haploid （DH） population derived form the cross between flue-cured tobacco cultivars Honghua Dajinyuan and Hicks Broad Leaf was used in phenotypic survey and SSR analysis. Six leaf yield-related traits, i.e., plant height （PH）, stem girth （SG）, internode length （IL）, leaf number （LN）, length of the largest waist leaf （LWL）, and width of the largest waist leaf （WWL）, were tested, which showed large correlations between each other. QTL mapping was carried out using the composite interval mapping method based a genetic map covering 1882.1 cM of tobacco genome with 611 SSR markers in 24 linkage groups. A total of 69 QTLs were detected, most of which exhibited small additive effects. Four QTLs associated with PH, IL, and WWL exhibited much higher phenotypic contributions than other QTLs, and a single locus explained 15-20% of the phenotypic variation. Many small regions were detected to harbor two or more closely linked QTLs for different traits. This result was in agreement with the correlation analysis of phenotypic traits.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第8期1407-1415,共9页
Acta Agronomica Sinica
基金
supported by Yunnan Tobacco Company(08A05,2010YN02,2011YN04)
China National Tobacco Company(110201002001)
关键词
烤烟
数量性状基因座
遗传作图
加倍单倍体
产量
Flue-cured tobacco （Nicotiana tabacum L.）； Quantitative trait locus （QTL）； Genetic mapping； Doubled haploid （DH）； Yield
