摘要
选择与羊同源性较高的牛RERGL基因组序列设计特异性引物,提取黔北麻羊脾脏总RNA,通过RT-PCR技术对RERGL基因进行克隆测序及序列分析。结果表明,成功克隆了黔北麻羊RERGL基因,其cDNA序列646 bp,GenBank登录号JN 671919,编码205个氨基酸。生物信息学分析表明,黔北麻羊RERGL基因蛋白二级结构α-螺旋占40.68%,延伸链占16.18%,无规则卷曲占43.14%;没有跨膜螺旋结构域且没有糖基化位点。
Primers of goat RERGL were design according to the homologic gene of ox. Total RNA of Qianbei Ma goat was extracted from the spleen. The cDNA sequence encoding RERGL was obtained by reverse transcription PCR （RT-PCR） and sequenced, The results demonstrated that the cDNA sequence of RERGL gene of Qianbei Ma goat was successfully cloned. The sequence length was 646 bp, encoding 205 amino acids; GenBank accession number was JN 671919. Bioinformatics analysis showed that the secondary structure of RERGL was 40.68% or-helix ＋16.18% extending chain＋43.14% random coil. No trans-membrane helix domain and O-glycosylation site was detected.
出处
《湖北农业科学》
北大核心
2012年第15期3362-3365,共4页
Hubei Agricultural Sciences
基金
贵州省农业科技攻关项目[黔科合N Y字(2008)3044]
