摘要
目的 观察并比较山莨菪碱与地塞米松对内毒素休克兔血压及血浆一氧化氮 (NO)水平的影响 .方法 采用静脉注射细菌内毒素复制内毒素休克模型 ,颈动脉 ,颈静脉插管法测定平均动脉压 (MAP)与中心静脉压 (CVP) ,用硝酸还原酶法加 Griess法测定血浆中 NO2 - 与 NO3- 的总含量间接反映 NO的水平 .结果 给予内毒素后 ,模型组的 MAP迅速下降 ,在低水平维持 ;CVP在 2 h后开始下降 ,4h后进一步下降 .山莨菪碱治疗组与地塞米松治疗组的 MAP未见明显下降 ,优于模型组 (P<0 .0 1) ;CVP无明显变化 ,与模型组差别显著 (P<0 .0 1) .注射内毒素 1h后模型组的血浆 NO生成水平进行性升高 ,相应的地塞米松治疗组 4h内未有明显变化 ,与模型组差异显著 (P<0 .0 1) .山莨菪碱治疗组的血浆 NO水平在 1h后也开始上升 (P<0 .0 5 ) ,但在 2 h时其 NO水平明显低于模型组 (P<0 .0 5 ) .结论 山莨菪碱能够明显改善内毒素休克兔的低血压状态 ,并对其
AIM To investigate effects of anisodamine on blood pressure and the synthesis of nitric oxide in endotoxic shock rabbits. METHODS Lipopolysaccharides (LPS) was injected into rabbits at the dose of 600 μg·kg -1 to build the endotoxic shock model. Carotid artery catheter and carotid vein catheter were used to monitor mean arterial pressure(MAP) and center venous pressure (CVP). The level of NO 2 - and NO 3 - in plasma was determined by using Griess reaction after reduction with nitrate reductase. RESULTS After being injected with LPS, the model's MAP decreased rapidly and retained low level all the time. The CVP also decreased after two hours, and came up to a lower level four hours later. No distinct changes of MPA and CVP were observed in anisodamine group and dexamethasone group; there was significant difference compared with the MPA and CVP of model group ( P <0.01). One hour after being injected with LPS, the level of NO 2 - and NO 3 - in plasma increased rapidly in model. At the same time, the level of NO 2 - and NO 3 - in dexamethasone group had no changes ( P <0.01); in anisodamine group it was higher than control, but lower than model ( P <0.05). CONCLUSION Anisodamine can significantly improve the hypotension endotoxic shock rabbits, and inhibit the synthesis of nitric oxide in endotoxic shock.
出处
《第四军医大学学报》
2000年第7期S189-S191,共3页
Journal of the Fourth Military Medical University
