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pDC316-HGFK1病毒穿梭载体的构建及鉴定 被引量:1

Construction and Identification of pDC316-HGFK1 Plasmid
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摘要 [目的]构建携带人HGFK1基因的病毒穿梭载体pDC316-HGFK1。[方法]通过RT-PCR方法从人新鲜胎盘组织中扩增出HGFK1基因,构建pDC316-HGFK1病毒穿梭载体,经PCR、酶切、测序等方法进行鉴定。[结果]酶切、PCR及测序证实pDC316-HGFK1载体序列正确。[结论]成功构建pDC316-HGFK1病毒穿梭载体,可为后续病毒包装及对肝癌细胞的生物学影响研究奠定基础。
机构地区 台州医院
出处 《肿瘤学杂志》 CAS 2012年第7期516-519,共4页 Journal of Chinese Oncology
基金 浙江省自然科学基金项目(Y2007048) 浙江省科技计划项目(2009C33SA800006)
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