摘要
目的:建立高效液相色谱法测定围手术期患儿血浆瑞芬太尼浓度。方法:采用Hypersil CN柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.02 mol·L^(-1)磷酸二氢钾水溶液内含三乙胺0.02%(30:70);流速1.0 ml·min～;检测波长210 nm;进样量为20μl。结果:标准曲线在1.0～100.0μg·L^(-1)内线性关系良好(r=0.999 3)。最低检测浓度为1.0μg·L^(-1)。提取回收率(76.51±0.82)%。方法回收率99.66%～102.40%。日内和日间RSD均小于15%。2μg·L^(-1)组靶控浓度与实测浓度基本一致。结论:本方法快速、准确、灵敏、专一性好,适用于临床瑞芬太尼血药浓度检测和药动学研究。
Objective： To establish a sensitive method for the determination of remifentanil in plasma of perioperative children by HPLC. Method： The separation was performed on a Hypersil CN column（250 mm × 4.6 mm, 5 μm） with the mobile phase of 0.02 mol ·L-1 KH2PO：acetonitrile （70： 30） （ including 0.02% triethylamine） at a flow rate of 1.0 ml· min -l. The detection wavelength was 210 nm. Result： A good linearity was obtained over the range of 1.0-100.0μg· L-1 （ r = 0.999 3 ）. The extraction recovery was （76.51 ＋ 0.82） % and the method recovery was 99.66% -102.4%. The inter and intra-day RSDs were less than 15%. The measured concentrations were similar to the target plasma concentrations in 2 μg · L-1 remifentanil group. Conclusion： The method is rapid, sensitive and specific for the determination of remifentanil in clinical pharmacokinetic and therapeutic drug monitoring study.
出处
《中国药师》
CAS
2012年第7期990-992,共3页
China Pharmacist
