摘要
为了优化蛹虫草多糖超声提取工艺,采用响应曲面法,根据中心旋转试验设计,研究超声提取时间、提取温度和液料比等工艺条件对提取率的影响。结果表明:蛹虫草多糖超声提取最佳工艺条件为超声提取时间65min、温度80℃、液固比32mL/g、提取2次,提取率可达3.89%。
Response surface methodology was used to optimize polysaccharide ultrasonic-assisted extraction process from cultured C.militaris based on a central composite design（CCD）,the effects of extraction time,extraction temperature and ratio of material to liquid on the yield of polysaccharide were studied.The results showed that the optimum extraction condition was obtained as follows： ultrasonic-assisted extraction time 65 min,extraction temperature 80℃,ratio of material to liquid 32 mL/g and extraction times two,then the yield of polysaccharide was 3.889%.
出处
《贵州农业科学》
CAS
北大核心
2012年第8期187-190,共4页
Guizhou Agricultural Sciences
基金
中科院科技支青项目"高活性微孔草不饱和脂肪酸提取技术及其产业化"(082B371211)
国家基金项目"特种资源植物微孔草种群恢复机制及保护途径研究"(31070475)
关键词
蛹虫草
多糖
超声提取
响应曲面法
Cordyceps militaris
polysaccharide
ultrasonic-assisted extraction
response surface methodology
