摘要
目的探讨洛伐他汀（Lov）诱导子宫内膜癌细胞株HEC-1-A G1期同步化的方法及HEC-1-A细胞解除G1期同步化后的细胞周期进程。方法Kit-8细胞计数（CCK-8）法测定HEC-1-A细胞的倍增时间，采用10、20、30、40μmol／L的Lov作用1×细胞倍增时间，采用流式细胞术检测G1期细胞的比例，获得G1期同步化的最佳Lov浓度；采用最佳浓度作用HEC-1-A细胞，于作用后0．5×倍增时间-2×倍增时间内，每隔4h检测同步化程度，获得最佳浓度Lov作用下的最佳同步化时间；解除G1期同步化，每隔4h检测一次，研究HEC-1-A细胞解除G1期同步化后的细胞周期进程。结果CCK-8法检测显示HEC-1-A细胞的倍增时间约为24h；HEC-1-A细胞的最佳G1期同步化条件为：40μmol／L Lov作用28h，可获取G1期细胞（87．87±0．70）％；HEC-1-A细胞于解除G1期同步化后20h，获得最大量的S期细胞，为（33．58±0．62）％；同时G1期细胞比例达到最低，为（58．42±0．54）％。结论40μmol／L Lov作用28h，可获得最大量G1期细胞；解除G1期同步化后20h，S期细胞比例最高，G1期细胞比例达到最低，达到满意的G1期同步化效果。
Objective To investigate the effects of lovastatin on inducing G1 phase synchronization in HEC-1-A cells and examine the cell cycle progression after desynchronization. Methods The doubling time of HEC-1-A cells was detected by cell counting Kit-8 assay. To determine the best lovastatin concentration of G1 synchronization, HEC-1-A cells were treated with lovastatin at concentration of 10, 20, 30 and 40 μmol/L respectively for 1 × doubling time, and the cell cycle was detected by flow eytometry （FCM）. To determine the best period of lovastatin treatment to achieve G1 synchronization, HEC-1-A cells were treated with lovastatin at the best concentration for 0.5 × to 2 × doubling time, and the cell cycle was detected every 4 h using FCM. Furthermore, the cell cycle progress of HEC-1-A ceils after desynchronization was also observed. Results The doubling time of HEC-1-A cells was 24 h. Treated with lovastatin at concentration of 40 ×mol/L for 28 h achieved maximum G1 arrest （87.87±0.70） % in HEC-1-A cells. Minimum G1 phase （58.42±0.54） % and maximum S phase （33.58±0.62） % were observed after desynchronizing for 20 h. Conclusion Maximum G1 synchronization of HEC-1-A cells is induced by lovastatin at concentration of 40 ×mol/L for 28 h. The HEC-1-A cells show minimum Gl phase and maximum S phase after desynchronizing for 20 h.
出处
《肿瘤研究与临床》
CAS
2012年第8期505-507,511,共4页
Cancer Research and Clinic
基金
基金项目：国家自然科学基金（30772332）
广东省科技计划（20118031800056）
