摘要
目的:建立凤仙透骨草中的芦丁、槲皮素和山奈酚的HPLC含量测定方法。方法:采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱;以乙睛-0.1%冰乙酸为流动相进行线性梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长260 nm。结果:芦丁、槲皮素和山奈酚与其他组份的色谱峰均得到基线分离,加样回收率平均值分别为98.89%,98.39%,98.48%,RSD分别为2.05%,1.51%,1.89%。结论:该方法快速、准确、重复性好,可作为凤仙透骨草质量控制的有效方法。
Objective：To establish a HPLC method for the determination of eldrin,quertetin and kaempferol in Impatiens balsamina.Method：The determination was performed on a Diamonsil C18 column（4.6 mm×250 mm,5 μm）,with a gredient mobile phase of acetonitrile-0.1% glacial acetic acid;the flow rate was1.0 mL·min-1;column temperature was 30 ℃;the detection wavelength was at 260 nm.Result：The chromatographic peaks of eldrin,quertetin and kaempferol with other components were completely separated.The average recovery was 98.71%,98.96%,98.89%,RSD 2.13%,2.34%,2.38%.Conclusion：The method was rapid and accurate for quality control of I.balsamina,and laid foundations for further research and development.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期106-108,共3页
Chinese Journal of Experimental Traditional Medical Formulae
