摘要
[目的]研究不同配比激素对三岛柴胡悬浮细胞生长发育的影响。[方法]采用均匀设计试验方法,对三岛柴胡悬浮细胞培养基的激素进行优化,并以最优激素配方为基础拟合三岛柴胡悬浮细胞培养中鲜重、干重与培养时间的关系曲线。[结果]三岛柴胡悬浮细胞培养的最优激素配方为:N6+0.5 mg/L NAA+0.75 mg/L 6-BA+1.5 mg/L KT;细胞鲜重与培养时间的关系拟合方程为Y=5.703 6X2-41.429X+136.44;细胞干重与培养时间的关系拟合方程为Y=2.021 8X2-22.595X+57.19。[结论]试验为研究三岛柴胡细胞和实现其大规模培养奠定了基础。
[ Objective] To study the effects of different hormones the suspension cell growth and development Of Bupleurum falcatum L.. [ Method] The plant growth hormone of medium for Bupleurum Falcatum L. suspension cell cultures was optimized by the test method of uniform design. [ Result] The optimal hormone formulation of B. falcatum suspension eel/cultures was N6 ＋ 0.5 mg/L NAA ＋ 0.75 mg/L 6-BA ＋ 1.5 mg/L KT. Based on the optimal hormone formulation to fit the curves about the relationship of the cell fresh-weight t and the cell dryweight in incubation time, the fitting equation of the relationship between the cell fresh-weight and the incubation time was Y = 5.703 6X2 - 41. 429X ＋ 136. 44; the fitting equation of the relationship between the cell dry-weight and the incubation time was Y = 2. 021 8X2 - 22. 595X ＋ 57.19. [ Conclusion] This study can lay the foundation for the further study and the large-scale culture about the B. falcatum L..
出处
《安徽农业科学》
CAS
2012年第26期12864-12865,12895,共3页
Journal of Anhui Agricultural Sciences
基金
黑龙江省自然科学基金资助项目(C201013)
